摘要
首先采用葡萄糖与精氨酸水热合成碳量子点,并研究了碳量子点的结构和光谱特性。然后使用激光微量热泳动技术(MST)研究碳量子点与4种三磷酸脱氧核苷酸分子(dNTP)和单双链DNA间的亲和行为。实验结果表明制备的碳量子点粒径均一、具有良好的荧光性等,碳量子点与dNTP和MST-1/2ssDNA间存在着亲和行为,而碳量子点与dsDNA间的亲和力不明显;同时发现碳量子点与dNTP和ssDNA分子间通过氢键形式结合,其结合强弱与dNTP分子结构及ssDNA碱基序列排布有关,而dsDNA分子结构稳定,没有多余氨基位点,与碳量子点无明显结合。
The carbon quantum dots （CDs） were synthesized via one-step hydrothermal processing using hybrid carbon source （glucose and arginine） and the properties of CDs were characterized by the modern test methods. The molecular interaction between CDs and dNTP （dATP, dTTP, dGTP, dCTP）, ssDNA or dsDNA were measured by the MST. The results show that with fine dispersity the CDs prepared by hydrothermal process are uniform in its size and good fluorescence characteristic. There had affinities between the CDs and dNTP or ssDNA, while there had no affinity between the CDs and dsDNA. The CDs are bond with dNTP in the form of weak hydrogen in water solution. The affinities were relative to species and amount of theses surface groups in CDs and the structures of the dNTP, and that CDs integrate with ssDNA by hydrogen and its strength was related to base sequence of ssDNA; while the affinity between the CDs and the dsDNA was very low, because of there were no extra sites in the structure of double-stranded DNA.
出处
《材料导报》
EI
CAS
CSCD
北大核心
2014年第20期1-5,共5页
Materials Reports
基金
973计划子项目(2011CB013000)
清华大学摩擦学国家重点实验室自主研究重点项目(SKLT10A02)
关键词
微量热泳动
碳量子点
DNA
亲和行为
microscale thermophoresis, carbon quantum dots, DNA, affinity
