摘要
试验比较了就巢鸡和产蛋鸡生殖系统发育及催乳素(PRL)和褪黑激素受体1c(Mel-1c)基因表达量的变化。从1296只42周龄商品代北京油鸡中挑选出有典型就巢行为表现的就巢鸡30只和无就巢行为表现的产蛋鸡30只,饲养在两个单独的栏舍内,每舍内15只就巢鸡和15只产蛋鸡,43周龄末对所有试验鸡采血测定血清PRL、促黄体素(LH)和雌激素(E2)含量,每舍内分别选取就巢鸡和产蛋鸡各3只进行屠宰,观察卵巢、输卵管发育情况,并取卵巢和输卵管样品,测定PRL和Mel-1c基因的相对表达量。结果表明:就巢鸡的PRL、LH浓度均显著高于产蛋鸡(P<0.05),而E2浓度显著低于产蛋鸡(P<0.05);就巢鸡和产蛋鸡在体重、卵巢发育方面有显著差异;就巢鸡卵巢中PRL基因、Mel-1c基因相对表达量显著高于产蛋鸡(P<0.05),而输卵管中不同生理时期基因相对表达量差异不显著(P>0.05);PRL基因相对表达量在卵巢中显著高于输卵管中(P<0.05),Mel-1c基因相对表达量在卵巢和输卵管中差异不显著(P<0.05)。
The reproductive system development and some related genes such as prolactin （PRL）and melatonin lc （Mel-lc） mRNA gene expression in broody hens and laying hens were compared in this paper. 30 laying hens having characteristic broody behavior and 30 without sign of broody behavior were chosen from 1 296 commercial Beijing You chicken （BYC） at 42 weeks of age,and housed in two separate pens. Each pen had 15 broody hens and 15 laying hens. At 43 weeks of age, all the hens were sampled and serum prolactin （PRL）,luteinizing hormone （LH）and 17-beta-oestradiol （E2） concentration were measured. 3 laying hens and 3 broody hens from each pen were slaughtered,respectively,the ovary and oviduct were observed and sampled,PRL and Mel-lc mRNA gene expression were determined. The results showed that serum PRL and LH concentration of broody hens were significantly higher than those of laying hens （P〈0.05）, E2 concentration were significantly lower than those of laying hens （P〈0.05）; There were significant differences in average body weight and ovary development between broody hens and laying hens （P〈0.05） ; The ovary PRL and MEL-lc mRNA gene expression in broody hens were significantly higher than those in laying hens （P〈0.05）, but there was no significant changes in oviduct; PRL mRNA gene expression in ovary was significantly higher than that in oviduct （P〈0.05）,Mel-lc mRNA gene expression had no differences between ovary and oviduct.
出处
《中国家禽》
北大核心
2014年第17期11-14,共4页
China Poultry
基金
国家自然科学基金项目(30972128
31372353)
