摘要
目的:通过对5,7-二甲氧基白杨素对HepG2细胞生长影响的研究,为寻找有开发前景的肝癌治疗活性新化学实体提供理论和实验依据。方法:通过MTT比色法测定观察对体外培养HepG2细胞和人胚肝(L-02)细胞增殖活性的影响,并评价其对人肝癌细胞作用选择性;细胞计数法观察活细胞及死细胞数,计算细胞存活率,绘制细胞生长曲线。结果:MTT比色法结果显示,以不同浓度的dMChR分别处理HepG2细胞48h,其IC50值为3.22μM,其中16.0μM的dMChR作用HepG2细胞48h的相对抑制率达79.59%,而相应浓度的ChR和5-FU的抑制率分别为35.28%和74.01%。且对HepG2细胞的增殖活性抑制作用选择指数达21.03;细胞计数显示:不同浓度的dMChR、5-FU和ChR均对体外培养HepG2细胞具有抑制生长作用,呈剂量依赖性,其作用效价高于ChR(P<0.05),而与5-FU类似;绘制生长曲线发现dMChR显著抑制HepG2细胞的生长,呈时间依赖性,其抑制作用强于先导化合物ChR,而与5-FU相似。结论:dMChR抑制HepG2细胞增殖活性作用强,对L-02细胞增殖活性抑制作用弱,其抑制HepG2细胞增殖活性作用具有相对选择性。
Obj ective：To provide theoretical and practical basis for the seeking of the promising new chemistry entities to the treatment of liver cancer by studing the effects of 5,7-dimethoxy-chrysin on HepG2 cells growth.Methods：The MTT colorimetric method was used to observe the inhibition effect the 5,7-dimethoxychrysin to the growth of HepG2,and evaluate its selectivity to the L-02;The cell counting method was used to observe the numbers of the living cells and died cells,calculate the cell survival rate and draw its growth rate.Results：The MTT assays result showed that after treating HepG2 with different concentrations of dMChR after 48 hours,the value of IC50 was 3.22μM and the relative inhibitory rate of the 16.0μM dMChR to the HepG2 after 48 hours was 79.59%,while the respective inhibitory rate of ChR and 5-FU was 35.28% and 74.01%,respectively.The selection index of the inhibitory effect to the proliferation activity of the HepG2 cells was 21.03.The cell counting methods showed that dMChR,5-FU and ChR of different concentrations can inhibit the growth of HepG2, with a dose-dependent manner.The effective titer was more then ChR（P〈0.05）and was similar with 5-FU.The growth rate showed that dMChR can obviously inhibit the growth of HepG2 with a time-dependent manner.Its inhibitive effect was stronger than the lead compound ChR and was similar with 5-FU.Conclusion：The inhibitive effect of dMChR to the proliferation activity of HepG2 cell was strong,while the effect was weak to the L-02 cell,Its inhibitive effect to the proliferation activity of HepG2 cells was relatively selective.
出处
《长治医学院学报》
2014年第4期241-244,共4页
Journal of Changzhi Medical College
基金
湖南省2011教育厅科技计划项目(11C1092)
衡阳市2011科技项目(KS10)
