摘要
目的探讨三七总皂苷（panaxnonginsengsaponins,PNS）对大鼠肝缺血再灌注损伤的保护作用,并研究其对氧化应激及炎症反应相关因子的影响。方法 30只健康雄性SD大鼠,体质量250～300g,随机分为假手术组（Sham组）、肝缺血再灌注组（HIR组）和三七总皂苷治疗组（PNS组）,各10只。HIR组和PNS组构建肝缺血再灌注模型,PNS组术前1h予50mg/kg PNS生理盐水溶液（5mg/mL）腹腔注射,Sham组及HIR组于术前1h给予相同剂量的生理盐水腹腔注射。预处理3h后处死大鼠,检测并比较三组肝组织丙二醛（MDA）、超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）、细胞间黏附因子-1（ICAM-1）及血清肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）水平。结果 PNS组肝缺血再灌注损伤后肝组织中MDA水平、MPO活性、ICAM-1含量及血清TNF-α、IL-1β低于HIR组（P〈0.05）,SOD活性则高于HIR组（P〈0.05）。结论三七总皂苷可通过减少氧化应激水平和炎症因子表达,减轻肝缺血再灌注损伤,达到肝脏保护作用。
Objective To investigate the protective effect of Panaxnonginsengsaponins（PNS） on hepatic ischemia reperfusion injury（HIR） in rat and explore the mechanism. Methods Thirty SD rats were divided to three groups：sham group,HIR group,and PNS group. The model of HIR was established in HIR and PNS groups. Rats in PNS group were given 50 mg/kg PNS solution（5mg/ml） by intraperitoneal injection at 1h before surgery. Other two groups were administered with equivalent saline. After 3 h from pre-injection, all rats were sacrificed to detect liver malondialdehyde（MDA）,superoxide dismutase（SOD）,myeloperoxidase（MPO）,intercellular adhersion molecule-1（ICAM-1）and serum tumor necrosis factor α（TNF-α）and interleukin 1β（IL-1β）. Results Compared with HIR group,the levels of liver MDA,MPO,ICAM-1 and serum TNF-α and IL-1β were higher in PNS group,while SOD was increased（P〈0.05）. Conclusion PNS can protect rats＇ liver against ischemic injury by deceasing oxidative injury and expression of inflammatory factors.
出处
《浙江中西医结合杂志》
2014年第8期665-668,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
关键词
大鼠
肝脏缺血再灌注
三七总皂苷
保护作用
rats
hepatic ischemia reperfusion
panaxnonginsengsaponins
protective effect
