摘要
目的观察感染后肠易激综合征模型小鼠肠道Cajal间质细胞改变对肠道动力及内脏敏感性的影响。方法 34只雌性C57BL/6小鼠随机分为模型组和对照组,每组17只。模型组用0.2 mL含400条旋毛虫幼虫的生理盐水悬液灌胃,对照组给予等量生理盐水灌胃。于感染后第14、28和56日,取回肠末端和近端结肠组织进行病理学检查。感染后第56日,检测小鼠腹壁撤退反射(AWR)评分、肠道传输时间、每2 h的粪便粒数和Bristol评分;免疫组织化学染色和RT-PCR测定不同肠段Cajal间质细胞中c-kit蛋白和mRNA的表达。结果感染后第56日,模型组小鼠肠道炎症完全消退;与对照组比较,模型组小鼠AWR评分显著增高(P<0.01),肠道传输时间明显缩短(P<0.01);模型组小鼠每2 h的粪便粒数、湿重和Bristol评分均显著高于对照组(P<0.05)。免疫组织化学染色和RT-PCR检测结果显示:与对照组比较,模型组小鼠各肠段Cajal间质细胞中C-kit蛋白和mRNA表达均显著增强(P<0.05)。结论感染后肠易激综合征小鼠肠道Cajal间质细胞改变可能是肠道动力紊乱和内脏敏感性增高的机制之一。
Objective To observe the effects of changes of interstitial cells of Cajal from mice with postinfection irritable bowel syndrome （PI-IBS） on the intestinal motility and visceral sensitivity. Methods Thirty four female C57BL/6 mice were randomly divided into the model group （n =17） and control group （n =17）. Mice of the model group were intragastricly injected by normal saline of 0.2 mL with 400 larvae of trichina. Mice of the control group were intragastricly injected by normal saline of the same volume. Pathological examinations were conducted for tissues of terminal ileum and proximate colon 14, 28, and 56 d after infection. The abdominal withdrawal reflex （AWR） scores, intestine transportation time, fecal grain numbers for every 2 h, and Bristol scores were determined 56 d after infection. The expressions of c-kit protein and mRNA in interstitial cells of Cajal of different intestinal segments were detected by the immunohistochemical staining and RT-PCR. Results The intestinal inflammatory reaction of mice of the model group regressed completely 56 d after infection. Compared to the control group, the AWR scores of mice of the model group were significandy higher （P〈0.01） and the intestine transportation time was significantly shorter （P〈0.01）. Grain numbers for every 2 h, wet weights, and Bristol scores of the model group were significantly higher than those of the control group （P〈0.05）. Results of the immunohistochemical staining and RT-PCR showed that compared to the control group, the expressions of c-kit protein and mRNA in interstitial cells of Caja1 of each intestinal segment of mice ofthe model group were significantly increased （P〈0. 05）. Conclusion The changes of the interstitial cells of Cajal of mice with PI-IBS may be one of the mechanisms of intestinal motility disorders and visceral hypersensitivity.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第7期978-983,共6页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81160057)~~
关键词
感染后肠易激综合征
CAJAL间质细胞
肠道动力
内脏敏感性
postinfection irritable bowel syndrome
interstitial cells of Caja1
intestinal motility
visceral sensitivity
