摘要
目的研究孕期多环芳烃暴露对新生儿神经行为发育的影响以及胎盘P300的变化,了解其是否可以作为一种效应标志物。方法收集孕产妇的尿液及其相应的胎盘(胎儿面)组织,用高效液相色谱法(HPLC)和酶联免疫吸附测定(ELISA)法分别测定尿中1-羟基芘和胎盘中P300的含量,运用20项神经行为测试法(NBNA)测定新生儿神经行为功能,计算NBNA总分,比较两地胎盘中P300含量的差异。结果共有116例新生儿胎盘组织符合条件,太原组和长治组的P300结果差异有统计学意义(P<0.05),太原组P300含量高于长治组,P300含量与尿中1-羟基芘含量呈正相关(r=0.202,P<0.05)。结论 P300的含量与孕期多环芳烃暴露对新生儿神经行为发育的影响有关联,但P300作为孕期多环芳烃暴露对新生儿神经行为发育造成影响的特定生物标志物仍需进一步研究。
[ Objective ] To study the effect of PAHs exposure during pregnancy on neonatal neurobehavioral development and the change of placental P300, understand weather p300 can serve as a effective biomarker. [ Methods ] Maternal urine and placenta tis- sue （ sub-surface } were collected. Urine 1-Hydroxypyrene and the content of P300 were determined by HPLC and ELISA respec- tively, and neonatal neurobehavioral function was measured by 20 neurobehavioral test method （NBNA）. The NBNA score was calculated and the difference of P300 content in two places was compared. [ Results] A total of 166 eases of neonatal placenta tissues accorded with the condition. The P300 content of Taiyuan group was higher than that of Changzhi group, and the difference was statistically significant （ P 〈 0.05 ）. The P300 content was positively correlated with urine 1-Hydroxypyrene （ r = 0.202, P 〈 0.05）. [ Conclusion] P300 content may be related to the effect of PAHs exposure during pregnancy on neonatal neurobehavioral development, but the P300 as a effective biomarker of effect of PAHs exposure during pregnancy on neonatal neurobehavioral development need further study.
出处
《职业与健康》
CAS
2014年第16期2242-2244,共3页
Occupation and Health
基金
国家自然科学基金(项目编号:30800899)
