摘要
建立了高效液相色谱法（HPLC）测定复方陆朴微乳剂中厚朴酚与和厚朴酚含量的方法。样品经85℃水浴去除溶剂后，加入甲醇溶解超声处理30min、过滤，取滤液，测定其中厚朴酚与和厚朴酚的含量。色谱条件：采用ZORBAXSB-C18色谱柱（250mm×4．6mm，5μm），流动相为0．5％磷酸-甲醇、流速为0．70mL／min、检测波长为294nm、柱温30℃，进样量10μL。结果表明：厚朴酚在0．074～0．518μg／mL（r=0．9997）、和厚朴酚在0．152～1．064μg／mL（r=0．9998）范围内，其质量浓度和峰面积之间均呈良好的线性关系；厚朴酚的添加回收率在98．8％-100．2％之间，相对标准偏差（RSD）为0．70％～1．O％（n=6）；和厚朴酚的添加回收率在98．5％～99．5％之间，RSD为0．90％～1．1％（n=6），5个不同批次的复方陆朴微乳剂中厚朴酚、和厚朴酚的平均含量分别为2．6924和1．2165mg／mL。该方法操作简便、准确度高、重现性好，可作为复方陆朴微乳剂中厚朴酚与和厚朴酚含量的测定方法。
A high performance liquid chromatography （HPLC） method was developed for determinating contents of magnolol and honokiol in Lupo compound microemulsion formulation. Samples were extracted with methanol for 30 minutes after them were evaporated by solvent completely at temperature of 85 ℃, obtaining the filtering filtrate for determination. The chromatographic conditions were as follow： ZORBAX SB-C18 （250 mm× 4. 6 mm, 5 μm） was used with the mobile phase of 0. 5% phosphoric acid-methanol and the flow rate was 0.70 mL/min, at the detectionwavelength of 294 nm, and the column temperature was 30 ℃. The results showed that the linear relationship of the magnolol density and peak areas was in the range of 0. 074 - 0. 518 μg/mL （ r = 0. 999 7 ）, and that of the honokiol was in the range of 0. 152 - 1. 064 μg/mL （ r = 0. 999 8 ）. The average recovery of magnolol was from 98.8% to 100. 2% with RSD from 0. 70% to 1.0% （n =6）, and that of honokiol was from 98.5% to 99.5% with RSD from 0. 90% to 1.1% （ n = 6）. In five different batches of Lupo compound microemulsion formulation, the average content of magnolol was 2. 692 4 mg/mL and that of honokiol was 1. 216 5 mg/mL. The method is simple,quick, accurate and reproducible and can be used to determinate the magnolol and honokiol contents in Lupo compound microemulsion formulation.
出处
《农药学学报》
CAS
CSCD
北大核心
2014年第4期427-432,共6页
Chinese Journal of Pesticide Science
关键词
复方陆朴微乳剂
厚朴酚
和厚朴酚
高效液相色谱
Lupo compound microemulsion formulation
magnolol
honokiol
high performanceliquid chromatography （HPLC）
