摘要
以亲水性离子液体1-丁基-3-甲基咪唑氯盐(BmimCl)为添加剂,研究离子液体对溶菌酶结晶的影响.分别考察了离子液体对溶菌酶晶体数量与尺寸、晶体形貌及蛋白质纯度的影响,并探讨了离子液体对结晶过程影响的作用机制.离子液体通过增大溶菌酶的溶解度和其自身低蒸气压两种途径,降低了溶菌酶在结晶过程中的过饱和度,更有利于晶体的成核和生长,得到更好的结果.如避免多晶态现象的发生,增大晶体的尺寸,降低溶菌酶样品纯度的要求.X-射线衍射分析表明,离子液体未改变晶体的晶型结构,但可提高晶体的衍射分辨率.
The crystallization of lysozyme with hydrophilic ionic liquid 1-buty-3-methyl- imidazoliumchloride （BmimC1） as an additive was investigated. We discussed the effects of ionic liquid on crystalnumber, crystal size, crystal morphology, the tolerance to concomitant impurities and the relevantmechanism for protein crystallization in the presence of ionic liquid. The elevated threshold to super-saturation caused by the increased solubility of lysozyme in the presence of BmimC1 and the slower super-saturation process of lysozyme induced by the negligible vapor pressure of BmimC1 contributed to a lowersuper-saturation degree, offering a promoted ambient circumstance for nucleation. These eventually offera prominent promotion to the crystallization of lysozyme, i. e, less crystal polymorphism and precipitationwhile larger crystals and significantly improved the tolerance to the concomitant impurities. X-raydiffraction analysis showed that the ionic liquid did not change the crystal structure, but it could improvethe resolution ratio of the crystal.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2014年第7期700-705,共6页
Chinese Journal of Biochemistry and Molecular Biology
基金
黄河水利职业技术学院校内基金(No.2011QNKY009)~~
