摘要
目的探讨长脉冲Nd:YAG 1 064 nm激光对临床分离不同表型红色毛癣菌形态学的影响。方法长脉冲Nd:YAG 1 064 nm激光分别以200 J/cm2、400 J/cm2、600 J/cm2能量体外照射含有等菌量的红色毛癣菌Y菌落(绒毛型)和红色毛癣菌N菌落(沟纹型),将激光照射前后的菌落制备成标本,分别在扫描电镜(SEM)及透射电镜(TEM)观察其形态结构变化。结果对绒毛型T.rubrum Y菌落,随着激光照射能量的增加,与对照组相比,绒毛型T.rubrum Y菌丝的形态结构破坏明显。表现为SEM下,菌丝结构变得粗糙、表面有凹陷、局部形成膨大,皱瘪非常明显;TEM下,菌体双层细胞壁及细胞器结构模糊或破坏,胞浆内可见脂滴和空泡变性,髓样小体及蛋白凝固体(为凋亡的表现),甚至菌体崩解、坏死。对沟纹型T.rubrum N菌落,随着激光照射能量的增加,与对照组相比,沟纹型T.rubrum N菌丝的形态结构破坏不显著。表现为SEM下菌丝结构变得弯曲、粗糙,表面有少量凹陷,菌丝局部膨大,无明显皱瘪;TEM下,菌体双层细胞壁及细胞器结构模糊或不连续,胞浆内可见脂滴和空泡变性,无凋亡及坏死表现。结论绒毛型红色毛癣菌Y菌落在激光照射后,其菌丝形态发生明显变化;而沟纹型红色毛癣菌N菌落在激光照射后,其菌丝形态变化相对较小。
Objective To explore the effect of long pulse Nd： YAG 1064 nm laser irradiation on morphological changes of Trichophyton rubrum Y（ T. rubrum Y） and Trichophyton rubrum N（ T. rubrum N）. Methods Equal number of colonies of T. rubrum Y（ phenotype villous） was irradiated in vitro by long pulse Nd： YAG 1 064 nm laser with different energy（ 0 J /cm2,200 J/cm2,400 J/cm2,600 J/cm2） and 0J /cm2for control group. The same procedure had been done for colonies of T. rubrum N（ phenotype furrowed） again. The above fungal samples were prepared before and after laser treatment in order to observe the morphological changes of T. rubrum Y and T. rubrum N by scanning electron microscopy（ SEM） and transmission electron microscopy（ TEM）. Results For villous colonies of T. rubrum Y,followed by upgrading laser energy treatment,the morphological feature of hyphae of T. rubrum Y had been severely destroyed. Under SEM,the structure of hyphae of villous T.rubrum Y became more rough,clearly bending,obviously shrunken and deflated. Furthermore many craters on the surface had been observed under SEM compared to control group. Under TEM,the structure of two layers of cell wall and cell organelle of T. rubrum Y became indistinct or discontinuous,and lipid droplet,vacuolar degeneration,myeloid body and albuminous degeneration（ these latter two performance indicate apoptosis）could be observed in Cysoplasm. Finally,irreversible necrosis happened. For furrowed colonies of T. rubrum N,followed by increasing laser irradiation,there were similar changes including rough surface with lightly bending,short swollen of hyphae. However,no obvious shrunken and deflated hyphae had been seen under SEM. TEM screening showed fuzzy or deformed two layers of cell wall,obscure or dissolved cell organelle,some lipid droplet and vacuolar degeneration were observed in T. rubrum N. No apoptosis or necrosis had been observed. Conclusion The obviously morphological changes were observed in villous colonies of T. rubrum Y after laser treatment. In contrast with these changes,the slightly morphological changes were observed in furrowed colonies of T. rubrum N after laser treatment.
出处
《临床和实验医学杂志》
2014年第14期1137-1140,共4页
Journal of Clinical and Experimental Medicine
基金
国家自然基金资助项目(81301385)
首都卫生发展科研专项项目(2011-1003-03)
