摘要
利用 3种不同来源的成纤维细胞 (大鼠肾上皮细胞 ,人胎肺成纤维细胞 ,人成纤维细胞 )的活细胞线粒体脱氢酶在电子偶联剂硫酸酚嗪甲酯 (phenazinemethosulfate,PMS)的协同作用下 ,还原四氮唑复合物 (XTT)形成可溶性的棕黄色甲簪 (formazan)产物 ,测定细胞甲簪的生成量来反映细胞的生长与活性状态 ,并与传统的四甲基偶氮唑盐 (MTT)方法作比较 .结果表明 ,XTT方法直接测定水溶性的甲簪产物 ,敏感度高于MTT法 ,具有操作简单、快速、灵敏度高、结果准确的优点 ,为成纤维细胞的研究建立了新的检测方法 .
In order to establish a sensitive method for detecting cellular growth of fibroblasts. Three kinds of fibroblasts were used, including normal rat kidney (NRK), human embryo lung fibroblast cell (MRC 5) and normal human fibroblast(NF). In those cells, mitochondrion dehydrogenase together with phenazine methosulfate (PMS) reduces 2,3 bis (2 methoxy 4 nitro 5 sulfophenyl) 5 [(phenylamino) carbonyl] 2H tetrazolium hydroxide (XTT) and a water soluble and light brown formazan product is formed. Cell growth and viability were obtained by measuring the metabolic product and compared with the traditional 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) method. It was shown that three kinds of cells have different metabolic response to XTT. XTT method directly detected water soluble formazan product. Its sensitivity was higher than MTT. These results indicate that it is a simple, rapid, sensitive and stable method. XTT colorimetric method establishes a new detective method for keloid research of traumatic surgery.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2002年第4期659-662,共4页
Progress In Biochemistry and Biophysics
