摘要
背景与目的:间隙连接在细胞间的营养物质、离子和细胞调节因子的交换中起重要作用。间隙连接异常,细胞间的物质交换障碍,往往导致细胞分裂失控。在有些癌组织和癌细胞中存在间隙连接的功能异常,恢复这些癌细胞的间隙连接功能,它们表现出正常细胞的生物学表型。因此,探讨细胞间隙连接蛋白在鼻咽癌中的表达,将有可能为临床诊断提供方法,为鼻咽癌的发病机理提供新思路。方法:采用免疫组化技术检测间隙连接蛋白Cx43、Cx45在鼻咽组织中的表达。结果:①Cx43、Cx45在鼻咽慢性炎症组织和鼻咽癌组织中有表达差异,在鼻咽癌中,Cx43、Cx45阳性率为44.8%、46.6%,与鼻咽慢性炎症柱状上皮细胞阳性率的86.5%和100%比较,显著性下降(P<0.01)。②鼻咽慢性炎症组织比鼻咽癌组织含鳞状细胞的百分率低(P<0.01),分别为29.7%和56.9%。③Cx43、Cx45在鼻咽癌旁柱状上皮细胞中的表达低于癌旁鳞状上皮细胞(P<0.001),高于癌细胞(P<0.01)。结论:Cx43、Cx45在鼻咽组织中的表达异常,可能与鼻咽组织鳞化和癌变有关。
Background and Objective: The gap junction plays an important role in the exchange of nutrients, ions,and regulatory molecules between cells. It will result in an uncontrolled cell proliferation if it is abnormal. It was reported that some cancer tissues and cancer cells had abnormal gap junction and restoration of the gap junction function could revert the cancer cells to a normal phenotype. Therefore, to investigate the expression of connexin(cx) in human nasopharyngeal carcinoma, may provide a new way for clinical diagnosis and the pathogenesis of nasopharyngeal carcinoma. Method: The expressions of Cx43 and Cx45 in the biopsies of nasopharyngeal carcinoma(NPC) and chronic nasopharyngitis tissues were determined by using the immunohistochemical staining. Results: 1. Cx43 and Cx45 expressed differentially in NPC and chronic nasopharyngitis tissues(P< 0.01). In NPC, expression of Cx43 and Cx45 were 44.8%and 46.6%, respectively, while in columnar cells of chronic nasopharyngitis tissues, their expressions were 86.5%and 100%respectively,which were higher than those in NPC, 2.The percentage in squamous epithelial cells of chronic nasopharyngitis tissues was 29.7%, which was lower than that in NPC,(56.9%). 3. The expressions of Cx43 and Cx45 in paracancer columnar epithelial cells were lower than that in paracancer squamous epithelial cells (P< 0.001), but higher than that in NPC cells (P< 0.01). Conclusions: The abnormal expression of Cx43 and Cx45 in nasopharynx tissues may be associated with cancerization and squamatization of human nasopharynx tissue.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2002年第6期593-596,共4页
Chinese Journal of Cancer
基金
国家863项目(No.102-10-01-05)
国家973重点项目(No.39700158)
