摘要
通过萃取、层析和固相萃取等方法预处理银杏叶提取物 ,然后用HPLC制备色谱柱自制银杏酸纯品 ,结合紫外光谱、氢核磁共振和高效液相色谱 ,对银杏酸进行结构解析。结果得到银杏酸Ⅰ、Ⅱ、Ⅲ 3种纯化合物 ,这 3种化合物的氢核磁共振所显示的官能团证实系银杏酸类化合物 ;并建立了HPLC法检测银杏叶提取物中的银杏酸的方法。
The sample of ginkgo biloba extracts was prepared by solvent extraction,column chromatography and solid phase extraction,the ginkgolic acids was purified by preparative HPLC.To analyse the molecule structures of ginkgolic acids by UV,H-NMR,HPLC.Finally three main compounds prepared from ginkgo biloba extracts are terrified to be ginkgolic acids through their functional groups by H-NMR,and a method for the determination of ginkgolic acids in ginkgo biloba extracts by HPLC is set up.
出处
《日用化学工业》
CAS
CSCD
北大核心
2002年第3期66-68,共3页
China Surfactant Detergent & Cosmetics
