摘要
蝗总科 8个科 10个种的线粒体DNA细胞色素b (Cytb)基因部分序列被测定分析 ,比较同源性 ,计算核苷酸使用频率并构建NJ分子系统树。在获得的 4 32bp的序列中 ,A +T约占 70 4 % ,其中 177个核苷酸位点存在变异(约占 4 1 0 % ) ,DNA一级序列数据显示 ,该 8科蝗虫间DNA序列变异丰富。用日本蚱作外群构建的NJ树表明 :瘤锥蝗科较为原始 ,而癞蝗科与槌角蝗科关系较近 ,为比较进化的类群 ,蝗总科 8科的起源关系为 :瘤锥蝗科→斑翅蝗科和锥头蝗科→斑腿蝗科→剑角蝗科→网翅蝗科→槌角蝗科和癞蝗科 ,上述研究结果与传统形态分类学结论不完全一致 。
The mtDNA Cyt b sequences (432bp) were analyzed in 10 individuals from 8 different families of Acridoidea in China. The homologous sequences were compared, the used frequency of nucleotide was calculated and the molecular phylogenetic tree constructed by Neighbor Joining method using T.japonica as outgroup. The confidence of nodes in the trees was evaluated by bootstrap(1000 replicates). These sequences were the middle part of Cyt b gene, and in the obtained sequences, A%+T% was about 70 4% and G%+C% only 29 6%. The sequence data revealed considerable variation in 177 nucleotide sites (about 41 0%) among the analyzed individuals from 8 different families. From every amino acid codon, A%+T% in the third site was higher (86 6%) than the other two sites and lower than other insects in the corresponding region. The NJ tree suggested that 11 individuals from the 8 families clustered in 4 groups, among which Gomphoceridae and Pamphagidae firstly clustered and then together with Arcypteridae and Acrididae to form group Ⅰ; Cluster Ⅱ was made of three species from Catantopidae, O.japonica, O.chinensis and O.intricata; Pyrgmorphidae and Oedipodidae formed group Ⅲ and Chrotogonidae single as cluster Ⅳ, respectively. The phylogenetic relationships of the 8 families was: Chrotogonidae→Pyrgomorphidae and Oedipodidae→Catantopidae→Acrididae→Arcypteridae→Gomphoceridae and Pamphagidae, which is little different from the morphological results.
基金
国家自然科学基金资助项目 (3 0 0 70 112 )
山西省自然科学基金资助项目 (9910 96) ~~
