摘要
为了验证及进一步研究促卵泡素受体(follicle-stimulating hormone receptor,FSHR)基因在绵羊繁殖力中的作用机制,试验采用生物信息学方法分析了绵羊FSHR基因结构、FSHR蛋白性质和结构、同源性、遗传进化、信号通路、蛋白互作网络和器官表达规律。结果表明:绵羊FSHR DNA全长196 149 bp,而编码蛋白质的mRNA序列全长只有2 431 bp,FSHR基因含有10个外显子;FSHR基因编码695个氨基酸,FSHR蛋白属于不稳定的脂溶性蛋白;FSHR蛋白是有7个跨膜结构的膜受体,包含LRR_5、GnRH_trans、7tm_1三种功能结构域,这三种结构域分别行使不同功能,共同激活G蛋白偶联机制,激活腺苷酸环化酶,使细胞内CAMP或Ca^(2+)内流增加,从而对细胞的生命活动进行调节;FSHR基因不但与繁殖相关基因Gdf9、Bmp15有关,还与抑制肿瘤转移基因Kiss1有关;FSHR的同源性分析和进化树构建结果显示,绵羊和山羊亲缘关系最近,其次是牛,与斑马鱼亲缘关系最远;FSHR基因在绵羊睾丸和卵巢中特异性高表达,并在各物种间的表达具有一致性。说明FSHR基因表达可能受内含子极为复杂和重要的调控,且在繁殖中起重要作用。该基因在睾丸中特异性高表达,与促卵泡生成素(FSH)对雄性动物曲细精管的发育、成熟和精子生成作用相吻合。
In order to verify and further study the mechanism of FSHR gene in sheep fertility,the bioinformatics method was used to analyze the genetic structure,protein structure and physicochemical properties,homology,genetic evolution,signal pathway,protein interaction network and tissue expression pattern of sheep FSHR. The results showed that the length of sheep FSHR DNA was 196 149 bp,while the full length of mRNA encoding protein was only 2 431 bp. The FSHR gene contained 10 exons,the FSHR gene encoded 695 amino acids,and the FSHR protein was an unstable liposoluble protein. FSHR protein was a membrane receptor with seven transmembrane structures. It contained three types of functional domains LRR_5,GnRH_trans,and 7tm_1. These three types of domains respectively performed different functions and activate the G protein coupling mechanism to activate adenylyl cyclase to increase intracellular CAMP or increase Ca2+ influx,which regulated the life activities of the cell. The FSHR gene was not only associated with the reproduction-related genes Gdf9 and Bmp15,but also related to the tumor metastasis suppressor gene Kiss1. Homology analysis and phylogenetic tree construction of FSHR showed that sheep and goats had the closest relationship,followed by cattle. And it was farthest from zebrafish. The FSHR gene was highly expressed specifically in the testes and ovaries of sheep and was conservative among species. The result suggests that FSHR gene expression may be regulated by introns,the process is very complex and important. The high expression of this gene in testis coincides with FSH promoting the development,maturation and spermatogenesis of seminiferous tubules in male animals.
作者
胡斐
吕慎金
金一
潘章源
HU Fei;LV Shenjin;JIN Yi;PAN Zhangyuan(Yanbian University,Yanji 133000,China;Linyi University,Linyi 276000,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2019年第3期24-27,31,177,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31572448
31272480)
临沂大学博士启动基金项目(40617036)
关键词
绵羊
促卵泡素受体基因
生物信息学
蛋白理化性质
蛋白互作
组织表达
sheep
FSHR gene
bioinformatics
protein physical and chemical properties
protein interaction
organizational expression
