摘要
目的探讨脂肪因子Chemerin对银屑病(psoriasis)外周血辅助性T细胞17(Th17)和调节性T细胞(Treg)的免疫调节作用。方法选取30例银屑病患者和28例健康对照者,应用磁珠分选法分离出外周血中的CD4^+T细胞。流式细胞术检测CD4^+T细胞中Th17和Treg细胞的比例。ELISA检测外周血中Chemerin的浓度。用不同浓度的Chemerin(50、100、150、200 ng/mL)处理健康的CD4^+T细胞,MTT法检测细胞活力以筛选Chemerin最适浓度。ELISA测定细胞上清中IL-17、IL-6、IL-10和TGF-β的含量。结果与健康对照组相比,银屑病组中的Th17细胞水平显著提升,Treg细胞水平明显降低,Chemerin的含量显著增大(P <0. 05)。用无毒性作用150 ng/mL的Chemerin处理健康的CD4^+T细胞,与对照组相比,150 ng/mL Chemerin组中IL-17和IL-6的含量显著升高,IL-10和TGF-β的含量降低,Th17的细胞水平提升,Treg的细胞水平降低(P <0. 05)。结论Chemerin能够通过提升Th17和抑制Treg细胞水平参与银屑病的发生发展。
Abstract:Objective To investigate the immunomodulatory effects of adipocytokine Chemerin on T helper cell 17(Th17)and regulatory T cell(Treg)in psoriasis.Methods Thirty psoriasis patients and 28 healthy controls were selected,and CD4+T cells from peripheral blood were separated by magnetic bead selection method.The percentage of Th17 and Treg cells were detected by flow cytometry method,the concentration of Chemerin was measured by Elisa method.The healthy CD4+T cells were treated with different concentrations of Chemerin(50,100,150,and 200 ng/mL),and MTT method was used to measure cell viability to select the optimal concentration of Chemerin.The levels of IL-17,IL-6,IL-10 and TGF-β were measured by Elisa method.Results Compared with the healthy control group,the level of Th17 was significantly increased,while that of Treg was significantly decreased.In addition,the level of Chemerin was obviously elevated in psoriasis group(P<0.05).The healthy CD4+T cells were treated with 150 ng/mL Chemerin.Compared with control group,the level of IL-17 and IL-6 were significantly increased,the level of IL-10 and TGF-β were decreased,the level of Th17 was promoted and the level of Treg was inhibited in 150 ng/mL Chemerin group(P<0.05).Conclusion Chemerin is involved in the development and progression of psoriasis by elevating Th17 and inhibiting Treg cells.
作者
王媛
张鼎伟
霍佳
胡刚
吴佳纹
WANG Yuan;ZHANG Dingwei;HUO Jia;HU Gang;WU Jiawen(Department of Dermatology,the Second Affiliated Hospital of Xi'an Jiaotong 710004,China)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2019年第2期138-142,共5页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金项目(81803161)
