摘要
目的探讨非小细胞肺癌组织中T细胞免疫球蛋白区黏蛋白-3 (TIM-3)、程序性死亡因子1 (PD-1)、程序性因子配体1 (PD-L1)的表达情况与临床意义。方法采用免疫组化SP法检测张家港市第一人民医院与苏州大学附属第二医院60例非小细胞肺癌与10例癌旁正常肺组织中TIM-3、PD-1、PD-L1的表达,分析其与临床病理特征及预后的关系,明确三者在非小细胞肺癌组织中表达的相关性。结果 60例非小细胞肺癌组织中,TIM-3、PD-L1的阳性表达率分别为51.67%、61.67%,PD-1在癌间质淋巴细胞的表达分为高密度组与低密度组,分别为24例、36例,10例正常肺组织中均未见TIM-3、PD-1、PD-L1阳性表达,与肺癌组的表达差异有统计学意义(P<0.05)。TIM-3、PD-L1的表达与淋巴结转移、肿瘤细胞分化程度、TNM分期及生存期有关(P<0.05),而PD-1的表达在各临床指标中未见相关性(P>0.05)。PD-1与PD-L1的表达呈正相关(P<0.05),而TIM-3与PD-1、TIM-3与PD-L1之间未见相关性(P>0.05)。Cox多元回归分析显示,TIM-3、PD-L1的高表达为患者生存期的独立影响因素(P<0.01)。结论 TIM-3、PD-1、PD-L1表达上调,在非小细癌胞肺癌的发展过程中具有重要作用,TIM-3、PD-L1可能成为监测非小细胞肺癌进展及预后的有效指标,阻断TIM-3、PD-1/PD-L1有望成为非小细胞肺癌治疗的新途径。
Objective To investigate the expression and clinical manifestation of T cell immunoglobulin region mucin-3(TIM-3), programmed death-1(PD-1) and programmed death-ligand 1(PD-L1) in non-small cell lung cancer(NSCLC) tissues. Methods The expression of TIM-3, PD-1 and PD-L1 were detected by immunohistochemical SP method in 60 cases of NSCLCs and 10 cases of adjacent normal lung tissues from Zhangjiagang First People’s Hospital and the Second Affiliated Hospital of Soochow University. The relationship of their expression with the clinicopathogical features and prognosis was analyzed, and the correlation between the expression of TIM-3, PD-1, PD-L1 in NSCLCs tissues was clarified. Results In 60 cases of NSCLCs tissues, the positive expression rates of TIM-3 and PDL1 were 51.67% and 61.67%, respectively. The expression of PD-1 in cancerous interstitial lymphocytes was divided into a high density group and a low density group, with 24 cases and 36 cases respectively. In 10 cases of normal lung tissues, there was no positive expression of TIM-3, PD-1 and PD-L1, and the difference was statistically significant compared with the expression of NSCLCs group(P<0.05). The expression of TIM-3 and PD-L1 had correlations with lymph node metastasis, differentiation of tumor cells, TNM stage and survival time(P<0.05), but no significant correlation was discovered between the expression of PD-1 and clinicopathological parameters(P>0.05). The positive correlation was found between the expression of PD-1 and PD-L1(P<0.05), while no correlation was considered between the expression of TIM-3 and PD-1(P>0.05). Multivariate analysis by Cox regression showed that the high expressions of TIM-3 and PD-L1 were independent prognostic factors in the survival of patients(P<0.01). Conclusion The up-regulated expression of TIM-3, PD-1 and PD-L1 plays an important role in the development of NSCLCs. TIM-3 and PD-L1 may be used as effective indicators for monitoring the progression and prognosis of NSCLCs. Blockage of TIM-3 and PD-1/PDL1 is expected to be a new approach for the treatment of NSCLCs.
作者
高锋
钱文霞
冯一中
GAO Feng;QIAN Wen-xia;FENG Yi-zhong(Department of Respiratory Medicine,Zhangjiagang First People's Hospital,Zhangjiagang 215600,Jiangsu,CHINA;Department of Pathology,the Second Affiliated Hospital of Soochow University,Suzhou 215004, Jiangsu CHINA)
出处
《海南医学》
CAS
2019年第2期154-158,共5页
Hainan Medical Journal
基金
江苏省张家港市科技计划项目(编号:ZKS1520)
