摘要
目的探讨罗格列酮对糖尿病大鼠胸主动脉钙化的影响。方法利用链脲佐菌素联合高能量饲料和维生素D3并尼古丁制备糖尿病血管钙化大鼠模型(DM+VDN组),并随机分为模型组、罗格列酮(RSG)治疗组和格列本脲(GLB)治疗组。检测大鼠一般代谢指标、血清糖基化终末产物(AGE)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,胸主动脉钙含量及糖基化终末产物受体(RAGE)蛋白表达。结果RSG治疗组和GLB治疗组血清AGE、MDA及SOD水平低于模型组,且RSG效果优于GLB(P<0.05);RSG治疗组胸主动脉RAGE表达水平以及钙含量均低于模型组和GLB治疗组(P<0.05)。结论罗格列酮可能通过降低AGE水平,下调RAGE表达,抑制氧化应激反应,延缓血管钙化进展。
Aim To elucidate effect of rosiglitazone ( RSG) on calcification induced by advanced glycation endproducts ( AGE) in vivo on rat. Methods A rat model of diabetic arterial calcification ( DM+VDN) was induced by streptozotocin ( STZ) and high fat diet,as well as vitamin D3 and nicotine,then divided into three groups: untreated group,group treated with RSG,group treated with glibenclamide ( GLB) . Metabolic parameters,aortic calcium content, malondialdehyde ( MDA) content,Cu /Zn superoxide dismutase ( SOD) activity,receptor for advanced glycation end products ( RAGE) and plasma AGE levels were measured. Results DM+VDN exhibited enhanced levels of AGE,as well as high levels of MDA and SOD. Aortas from DM+VDN exhibited high levels of calcium content. This calcification was also dramatically increased,as shown by von Kossa staining. In aorta,strong immunostaining for RAGE were observed in DM+VDN. Conversely,rosiglitazone attenuated these changes in calcium accumulation and the investigated proteins in aortas, as well as plasma AGE,MDA and SOD. Conclusion The results suggest that rosiglitazone might exert anti-calcification in partly through down-regulation of RAGE expression,thus limiting the cells’susceptibility toward oxidative stress induced by AGE.
作者
任晓妹
魏芹
刘乃丰
任利群
慕广建
李荥娟
REN Xiaomei;WEI Qin;LIU Naifeng;REN Liqun;MU Guangjian;LI Xingjuan(Zhongda Hospital of Southeast University,Nanjing,Jiangsu 210009,China)
出处
《中国动脉硬化杂志》
CAS
2018年第12期1201-1205,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金(81170254,81300227)资助
关键词
糖尿病
糖基化终末产物
糖基化终末产物受体
血管钙化
罗格列酮
diabetes mellitus
advanced glycation end-products
receptor for advanced glycation end products
calcification
rosiglitazone
