摘要
目的探讨葛根枳椇子栀子提取物(PHGE)解酒护肝作用。方法 SPF级Wistar雄性大鼠120只,随机分为对照组、模型组和PHGE低、中、高剂量(0.2、0.5、1.2 g/kg)组,每组24只。除对照组外,其他组别通过喂饲Regular型Lieber-DeCarli酒精液体模型饲料造模,造模过程中,PHGE各组动物ig不同剂量的PHGE。每周测定动物体质量1次,每天测定摄食量1次;给药4周和8周每组分别取半数动物禁食过夜,采血测定血清生化指标;解剖取肝脏称质量并计算脏体系数;取部分肝脏冰冻切片进行油红"O"染色,观察肝脏脂肪肝情况;测定肝脏总脂肪含量,测定肝脏组织超氧化物歧化酶(SOD)、丙二醛(MDA)、总谷胱甘肽(GSH)、乙醇脱氢酶(ADH)和乙醛脱氢酶(ALDH)。结果 4周和8周造模,大鼠体质量均增长减缓(P<0.05、0.01),第8周PHGE高剂量组动物体质量显著低于模型组(P<0.05);摄食量未见明显差异。造模4周,模型组血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇(TCHO)、高密度脂蛋白胆固醇(HDL)、低密度脂蛋白胆固醇(LDL)、血糖(GLU)显著高于对照组(P<0.05、0.01);与模型组比较,PHGE低、中、高剂量组AST、GLU,中、高剂量组ALT、TCHO、HDL,中剂量组LDL均显著下降(P<0.05、0.01),并呈明显的剂量相关性,造模8周与造模4周结果一致。造模给药8周,模型组GSH显著高于对照组(P<0.01),PHGE高剂量组GSH显著高于模型组(P<0.01),高剂量组ADH显著高于模型组(P<0.05);与对照组比较,造模给药4、8周,模型动物肝脏脂肪量显著升高(P<0.05、0.01),给药8周,PHGE高剂量组肝脏脂肪量显著低于模型组(P<0.05)。模型组肝脏质量与对照组比较未见显著性差异,而肝脏系数显著增加(P<0.01),应为动物体质量较小引起,PHGE各剂量组肝脏系数与同期模型组比较未见显著性差异。肝脏脂肪染色后,造模动物主要表现为弥漫性肝细胞内、中央静脉周边肝细胞内红染脂滴,PHGE中、高剂量组评分低于模型组。结论 PHGE发挥明显预防和/或改善酒精性脂肪肝的作用,改善肝功能,降低血糖和胆固醇。
Objective To study the efficacy of Puerariae Lobatae-Hovenia acerba-Gardeniae Fructus extractive(PHGE) for antialcohol and hepato-protection. Methods Rat model of alcoholic fatty liver was induced by feeding with 5% ethanol-containing Lieber-DeCarli liquid diet for 4 and 8 weeks. 120 SPF Wistar male rats were assigned into 5 groups, include vehicle control, model control and treatment groups which were 0.2, 0.5, 1.2 g/kg Gegen-Zhijuzi-Zhizi extract. Food consumption was detected daily. And body weight was detected weekly. Animals were sacrificed after overnight fasting at 4 and 8 weeks of study. Blood samples were collected for biochemical analysis. Livers were weighed, and prepared for histopathological examination with oil red O stain. The content of liver ADH, ALDH, GSH, SOD and MDA in rats were also determined. Result After 4 and 8 weeks, the body weight gained of rats slowed down(P < 0.05, 0.01). At 8 weeks, the body weight of rats in the high dose PHGE group was significantly lower than that of the model group(P < 0.05), and there was no significant difference in food intake. After 4 weeks, the levels of ALT, AST, TCHO, HDL, LDL and GLU in the model group were significantly higher than those in the control group(P < 0.05,0.01). Compared with the model group, AST and Glu in PHGE low, middle and high dose group, ALT, TCHO, HDL in middle and high dose group, and LDL of middle dose group were significantly decreased, and showed significant dose dependence. The results of 8 weeks and 4 weeks were consistent. After 8 weeks, GSH in model group was significantly higher than that in control group(P <0.01), GSH in high dose PHGE group was significantly higher than that in model group(P < 0.01), and ADH in high dose PHGE group was significantly higher than that in model group(P < 0.05). There was no significant difference in liver weight between the model group and the control group, but the liver relative weight increased significantly(P < 0.01), which should be caused by the small lower lever of animal weight. There was no significant difference in liver relative weight between the PHGE dose groups and the model group at the same time. After liver fat staining, diffuse hepatocytes and peripheral hepatocytes of central vein were the main manifestations of model animals. The score of middle and high dose PHGE group was lower than that of model group.Conclusions PHGE plays a significant role in preventing and/or improving alcoholic fatty liver, improving liver function, reducing blood sugar and cholesterol.
作者
梁金强
陶施民
余庆涛
卢贤欢
郭雅娟
葛亚中
马忠华
黄芝瑛
LIANG Jinqiang;TAO Shimin;YU Qingtao;LU Xianhuan;GUO yajuan;GE Yazhong;MA Zhonghua;HUANG Zhiying(School of Pharmaceutical Science,Sun Yat-Sen University,Guangzhou 510006,China;Guangdong Laboratory Animals Monitoring Institute,Guangzhou 510663,China;Infinitus (China)Co.Ltd,Guangzhou 510663,China)
出处
《药物评价研究》
CAS
2018年第11期1981-1988,共8页
Drug Evaluation Research
关键词
葛根枳椇子栀子提取物
酒精性脂肪肝
护肝
肝功能
脂代谢
血糖
肝脏系数
Puerariae Lobatae-Hovenia acerba-Gardeniae Fructus extractive
alcoholic fatty liver
hepatic protection
hepatic function
lipid metabolism
blood sugar
liver coefficient
