摘要
以重庆地区主栽水蕹菜(Ipomoea aquatica)为试材,在附加不同浓度配比的6-BA、IAA、NAA和2,4-D的MS培养基上离体培养,进行嫩茎高效无性系建立的研究。结果表明:1/2MS+6-BA 0.5 mg/L+2,4-D 1.5 mg/L培养基能诱导水蕹菜嫩茎产生大量的愈伤组织,其诱导率达93.3%;在继代培养中,诱导愈伤组织分化的最佳培养基为1/2MS+6-BA 0.5 mg/L,其分化率为92%;分化不定苗生根的最佳培养基为1/2MS+0.9 mg/L NAA;在无菌苗移栽培养(基质配方为草炭:菌渣:蛭石=1:1:1)中,幼苗株高为11.43 cm,且成活率达98%。
The paper aims to construct a highly efficient asexual system of Ipomoea aquatic,which is the main cultivated vegetable in Chonqing.The young stems were cultured on MS medium with 6-BA,IAA,NAA and 2, 4-D of different concentrations.The results showed that,lots of callus were induced in the medium of 1/2MS+ 6-BA 0.5mg/L+2,4-D 1.5mg/L,and the inducement ratio was up to 93.3%.In seedling subculture,the differentiation medium of callus induction was 1/2MS+6-BA 0.5mg/L,and the differentiation frequency was 92%.The medium of 1/2MS +0.9mg/L NAA was suitable for water spinach root growth.The substrate composition of peat,mushroom dregs and vermiculite (1:1:1)was suitable for aseptic seedlings growth,with shoot length of 11.43cm and the survival rate of 98%.
作者
陈磊
刘玉英
刘星
罗云米
Chen Lei;Liu Yuying;Liu Xing;Luo Yunmi(Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;Chongqing Jiulongpo Agriculture Committee,Chongqing 400051,China)
出处
《农学学报》
2018年第12期54-57,共4页
Journal of Agriculture
基金
重庆市九龙坡区科技计划项目"重庆水藤菜种苗早春快繁及栽培示范"(2017.15)
重庆市农发资金项目(NKY-2016AB007)
关键词
水蕹菜
无性系
无菌苗
Ipomoea aquatica
Asexual System
Aseptic Seedlings
