摘要
称取复方咪康唑乳样品约1g,加入乙醇25 mL,加热溶解后冷却,用乙醇定容至50mL,摇匀,冷冻1h后迅速用滤纸过滤,取续滤液放置至室温后,采用Agilent Eclipse Plus C18色谱柱(250mm×4.6mm,5μm)进行分离,以甲醇、乙腈和30g·L^(-1)乙酸铵溶液的混合液为流动相进行梯度洗脱,乳酸依沙吖啶、曲安奈德和硝酸咪康唑的检测波长分别为270,240,230nm。乳酸依沙吖啶、曲安奈德和硝酸咪康唑的线性范围分别为40～180,40～200,800～401mg·L^(-1),检出限(3S/N)分别为0.75,1.06,2.18mg·L^(-1)。方法用于测定复方咪康唑乳样品中的乳酸依沙吖啶、曲安奈德和硝酸咪康唑,测定值与标示值一致,加标回收率在96.1%～104%之间,测定值的相对标准偏差(n=6)小于1.0%。
The sample of compound miconazole cream(about 1g)was added into 25 mL of ethanol and heated for disolution.After cooling,the solution was made up to 50 mL with ethanol and mixed well.After freezing for 1h,the solution was filtrated immediately with filter paper.The filtrate was taken and cooled to room temperature.Agilent Eclipse Plus C18 column(250 mm×4.6 mm,5μm)was used as stationary phase for seperation,with a mixture of methanol,acetonitrile and 30g·L-1 ammonium acetate solution as mobile phase for gradient elution.The detection wavelengths for ethacridine lactate,triamcinolone acetonide and miconazole nitrate were 270,240,230 nm,respectively.The linear ranges of ethacridine lactate,triamcinolone acetonide and miconazole nitrate were 40-180,40-200,800-401mg·L^-1,and the detection limits(3S/N)were 0.75,1.06,2.18mg·L^-1,respectively.The method was used to determine ethacridine lactate,triamcinolone acetonide and miconazole nitrate in compound miconazole cream,giving results in contency with the marked values.Values of recovery obtained by standard addition method were in the range of 96.1%-104%,and RSDs(n=6)were less than 1.0%.
作者
肖丽丽
崇岚
曾国生
罗美兰
XIAO Lili;CHONG Lan;ZENG Guosheng;LUO Meilan(Dermatology Hospital of Jiangxi Province,Nanchang 330001,China;Food and Drug Inspection Team of Nanchang City,Nanchang 330038,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2018年第12期1415-1418,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
