摘要
采用共价接枝将CD34抗体接枝到聚乙二醇修饰的四氧化三铁磁性纳米粒子上,构建具有CD34抗体与PEG共同修饰的功能型磁性纳米粒子。通过红外检测(FT-IR)、纳米粒子粒径分析(动态光散射和透射电子显微镜)等方法对修饰后的磁性纳米粒子进行检测,并将其与内皮祖细胞(EPCs)共混培养,评价纳米粒子的细胞相容性以及对EPCs短时间的结合效果,然后在动态外加磁场的作用下评价修饰后磁性纳米粒子对EPCs的定向引导效果。结果表明,CD34抗体成功修饰到磁性纳米粒子表面,修饰后的纳米粒子在一定的浓度范围内有较好的细胞相容性,短时间对EPCs具有较好的识别结合作用,并且能在外加磁场作用下初步实现在动态环境下对EPCs的定向引导。
Immobilization of CD34antibody on ferroferric oxide magnetic nanoparticles was achieved by the traditional carboxyl-amine conjugation reaction.Fourier transform infrared spectroscopy (FT-IR),nanoparticle size analysis (dynamic light scattering and transmission electron microscope),and other testing methods were used to detect the surface modified magnetic nanoparticles.The endothelial progenitor cells (EPCs)were cultured with the surface modified magnetic nanoparticles to evaluate cell compatibility and the combination effect of nanoparticles on EPCs in a short period of time.Directional guide of the surface modified magnetic nanoparticles to EPCs was evaluated under applied magnetic field and simulated dynamic flow condition.The results showed that the magnetic nanoparticles were successfully modified with CD34antibody,which had good cell compatibility within a certain range of the nanoparticle concentrations.The surface modified nanoparticles can combine with EPCs effectively in a short time,and those nanoparticles combined EPCs can be directional guided under the magnetic field in the dynamic flow environment.
作者
范仪
杜泽煜
黄楠
FAN Yi;DU Zeyu;HUANG Nan(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,P.R.China;Key Laboratory of Advanced Technologies of Materials (KLATM),Ministry of Education,Southwest Jiaotong University,Chengdu 610031,P.R.China)
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2018年第6期900-904,913,共6页
Journal of Biomedical Engineering
基金
国家自然科学基金(81271701
31300792)
关键词
CD34抗体
四氧化三铁
内皮祖细胞
定向引导
CD34antibody
ferroferric oxide
endothelial progenitor cells
directional guide
