摘要
目的探讨不同免疫遗传背景对裸鼠成瘤性的影响。方法按照中国药典细胞成瘤性检测方法要求,对A、B两实验室裸鼠均作阴阳对照组处理,取脏器组织进行病理学分析;采集眼球血,应用流式技术分析相应的免疫指标;并分别提取鼠尾DNA用23对微卫星引物,对2群体进行微卫星分型。结果B实验室阳性对照不成立(出瘤7/10)。两实验室裸鼠病理检测无明显差异。A、B两实验室裸鼠在CD25、CD8、CD4、Th1、Th2五项免疫指标存在差异,分别检出13和18个多态位点。进一步对B实验室3个未出瘤个体进行比对,发现其免疫指标CD25、Th2和遗传标记D3Mit29、D5Mit48表现出高值与特征性带型。结论裸鼠免疫遗传背景差异与裸鼠成瘤性之间可能存在一定关联.
Objective To study the relationships between differences in tumorigenicity and immu-nogenetic backgrounds of nude mice. Methods According to the Chinese Pharmacopoeia, positive and neg-ative groups were set up in both Laboratory A and B with ten nude mice in each group. Organ tissues were collected for clinicopathological analysis. Blood samples were collected and detected using flow cytometry. DNA was extracted and analyzed with 23 STR markers. Results The positive group of Laboratory B was in-valid (7/10 tumor formation). The two laboratories showed no significant difference in the results of patho-logical analysis, but had significant differences in CD25, CD8, CD4, Th1 and Th2. There were 13 and 18 polymorphic sites respectively found in nude mice of Laboratory A and B. Further analysis of the non-tumor-bearing nude mice in Laboratory B positive group revealed that CD25, Th2, D3Mit29 and D5Mit48 were the specific indexes. Conclusion Differences in tumorigenicity might be related to the diversity of immunoge-netic backgrounds of nude mice.
作者
魏杰
王洪
樊金萍
范涛
梁春南
刘佐民
岳秉飞
Wei Jie;Wang Hong;Fan Jinping;Fan Tao;Liang Chunnan;Liu Zuomin;Yue Bingfei(Division of Laboratory Animal Monitoring,National Institute for Food and Drug Control,Beijing 102629,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2018年第11期835-840,共6页
Chinese Journal of Microbiology and Immunology
关键词
裸鼠
成瘤性
免疫
遗传
Nude mice
Tumorigenicity
Immune
Genetics
