摘要
将12窝杜洛克×长白×约克夏三元杂交新生仔猪随机分为4组,分别于17、21、28和35日龄断奶后,测定断奶前后胰腺组织和十二指肠、空肠、回肠的食糜及粘膜中胰糜蛋白酶的相对活性、比活和总活性。结果为:哺乳仔猪在断奶前胰腺糜蛋白酶活性保持较高水平,22日龄达到最高值,29日龄时明显下降（P<0.05）。而仔猪断奶后12h各组胰腺糜蛋白酶的相对活性和比活均显著提高,较同期哺乳仔猪高,随后降低,且在断奶后2～3周内均保持较低水平。18至50日龄间,哺乳仔猪及断奶仔猪胰腺中糜蛋白酶总活性在哺乳期和断奶后并没有随胰腺重量的增加而增加（P<0.05）。哺乳期空肠中糜蛋白酶活性的变化模式与胰腺的大体一致。若断奶日龄早于28日龄时,断奶后1周空肠中糜蛋白酶的活性降低。而28、35日龄断奶,断奶后这两种酶的活性不受影响,而且断奶日龄越早,降低后恢复至正常值的时间越长。十二指肠糜蛋白酶活性在断奶后的变化情况基本与空肠相似,但是回肠中却没能有规律地反映糜蛋白酶活性与日龄之间的关系。
Twelve litters of new born piglets were divided randomly into group Ⅰ 、 Ⅱ、Ⅲ and IV and weaned at 17, 21, 28 and 35 days of age, respetively, to determine the effect of weaning age on pancreatic and intestinal chymotrypsin activity in piglets. The result showed that the relative and specific activity of pancreatic chymotrypsin increased significantly 12h after weaning compared with that of sucking piglets at the same days of age and then decrased and remained low for 2 ~ 3 weeks after weaning. Total pancreatic chymotrypsin activity had no change with pancreas weight during the 18 days and 50 days of age regardless of weaning (P>0. 05). Chymotrypsin activity of jejunum had the same change as that of pancreas during sulking stage. Jejunum chymotrypin activity decreased 1 week after weaning in the groups weaned earlier than 28 days but no influence on them in groups weaned at 28 and 35 days. The earlier the weaning age, the longer the restoring time. Chymotrypsin activity in ileum is more sensitive to the effects of weaning age of piglets than in duodenum and jejunum.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第1期113-116,共4页
Scientia Agricultura Sinica
基金
"九.五"国家重点科技攻关项目成果(96-009-01-06)
