摘要
目的 :研究三七总甙 (PNS)对人肾成纤维细胞 (KFB)增殖、分泌I型胶原、表达整合素 β1的影响。方法 :体外培养KFB ,分别采用四甲基偶氮唑蓝 (MTT)法、ELISA法、流式细胞仪检测PNS对KFB增殖、分泌Ⅰ型胶原及表达整合素β1的影响。结果 :PNS在最佳浓度范围和作用时间内可明显抑制KFB细胞增殖及分泌Ⅰ型胶原 (与空白对照组比较 ,P <0 0 5 ) ,同时显著降低了KFB上整合素β1的表达 (与空白对照组比较 ,P <0 0 5 )。结论 :PNS可成为防治肾间质纤维化的有效药物。
To study the effect of Panax notoginseng saponi ns (PNS) on cell proliferation, type Ⅰ collagen secretion and integrin β 1 ex pression in human kidney fibroblast (KFB). Methods: KFB were cultured and stimulated by PNS in vitro. The cell proliferation, type Ⅰ collagen secretion and integrin β 1 expression in KFB were measured by methyl thiazolyl tetrazolium (MTT), enzyme linked immunoa bsorbent assay (ELISA) and flowcytometry respectively. Results: Within its optimal concentration range and effective time range, PNS could obviously inhibit the proliferation, type Ⅰ collagen secr etion and integrin β 1 expression (all P<0 05) in human KFB. Conclusion: PNS would possibly be an effective drug for renal interstitial fibrosis prevention and treatment.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第1期47-49,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
云南省卫生厅科研基金资助项目 (No .99M1 2 5)
