摘要
首次采用高效液相 蒸发光散射检测器色谱法测定桔梗〔Platycodongrandiflorum (Jacq .)A .DC .〕中桔梗皂苷D含量 ,以C18(5 μm ,4.6mm× 2 5 0mm)为色谱柱 ,V(乙腈 )∶V(水 ) =2 8∶72为流动相 ,流速 0 .7mL/min ,以ELSD 5 0 0为检测器 ,外标法定量。结果表明 :桔梗皂苷D在 0 3～ 2 4μg范围内呈线性 ,回收率为 99 5 7% (n =5 ) ,RSD为1 10 %。对 8种不同来源的桔梗材料和 2 0个四倍体株系桔梗皂甙D含量的测定说明 ,此法是测定桔梗皂苷D的较佳方法 。
This paper established a new HPLC-ELSD method to determine the content of platycodin D in different samples of Platycodon grandiflorum (Jacq.) A. DC. Alltech C\-\{18\}column (5 μm, 4.6mm×250mm) was used, mobile phase was V (acetonitrile)∶ V (water)=28∶72 at 0.7 mL/min speed. ELSD-500 detector was used for qualitative and quantitative analysis in external standard method.\ Obtained results indicated that within 0.3-2.4 μg platycodin D had a good linearity. The average recovery was 99.57% (n=5) with RSD 1.10%. In order to evaluate the reliability of this new method, the content of platycodin D in seven different original samples and 20 induced tetraploid lines in tissue culture were determined. The obtained results indicated that this is first time to report an advanced and effective method for the quality control and evaluation for raw materials of P. grandiflorum .
出处
《植物资源与环境学报》
CAS
CSCD
2001年第4期11-13,共3页
Journal of Plant Resources and Environment
