摘要
目的和方法:采用溶血试验、肝匀浆脂质过氧化产物丙二醛(MDA)含量测定、受照质粒DNA构象改变等为检测指标测定松树皮原花素对离休生物组织的抗氧化作用。结果:松树皮原花素能显著抑制H_2O_2所致的人红细胞(RBC)溶血作用(P<0.01),明显抑制VitC/Fe^(2+)诱导的小鼠肝匀浆组织过氧化作用(P<0.01),明显减轻^(60)Coγ射线所致质粒PUC_(18)DNA单链断裂程度(P<0.01)。结论:原花素具有很强的抗氧化作用,能保护离体RBC膜、DNA免受氧自由基的损伤。
Objective: To assess the antioxidative effect of procyanidins from pine bark on free radical damage. Methods: Hemolysis, Malonaldehyde(MDA) level of mice liver homogenates, the conformation changes of irradiated plasmid PUC18 were used as indexes. Results: Procyanidins could reduce the hemolysis degree of human RBC induced by H2O2 significantly(P < 0.01), reduce the MDA level of mice liver homogenate initiated by VitC/Fe2+ remarkably(P<0.01), and reduce the degree of single-strain break of plamid PUC18 induced by 60Coγ sig-nificantly(P<0.01).Conclusion: Procyanidins had good antioxidative function. It can prevent RBC membrane, plasmid DNA suffering from oxygen free radical damage in vitro.
出处
《中药材》
CAS
CSCD
北大核心
2001年第12期882-883,共2页
Journal of Chinese Medicinal Materials
