摘要
目的 :观测抑癌基因P1 5在原发性胃癌及转移淋巴结中的表达 ,以探讨其胃癌转移及预后的作用。方法 :应用免疫组化检测 92例原发性胃癌及 1 3 8枚转移淋巴结中P1 5蛋白的表达。用已知食管癌阳性切片对照。结果 :P1 5阳性表达率在原发癌中为 2 8.3 % ;原发癌伴淋巴结转移组P1 5阳性表达率较无淋巴结转移组显著低表达 (P <0 .0 0 5 ) ,转移组原发癌P1 5阳性表达率与转移淋巴结中的表达比较 ,两者有显著性差异 (P <0 .0 5 ) ,转移组原发癌P1 5阳性表达率与转移淋巴结中的表达比较 ,两者有显著性差异 (P <0 .0 5 )。结论 :P1
Objective:To investigate the expression of tumor suppressor genes P15 in human primary gastric carcinoma (PGC) and metastatic lymph node(MLN) and to explore the role of both expression in the carcinoma metastasis and the prognosis of PGC.Mothod:The expression of P15 in 92 cases of PGC and 138 MLN were detected by SP immunohistochemical method.All samples were obtained freshly,fixed with～10% buffered fomalin immediately,embedded with paraffin and then sectioned serilly.Using known positive staining section of P15 in esophgeal carcinoma as contrast.Results:The positive expression rates of P15 were 28.3% in PGC.The expression level of P15 in PGC with lymph node metastasis was significantly lower than that without lymph node metastasis (P<0.005).The positive of P15 in PGC with lymph node emtastasis was lower than that in MLN(P<0.05).Conclusions:The detection P15 protein may be in evaluation of lymph node metastasis and prognosis of PGC.
出处
《长治医学院学报》
2001年第3期163-165,共3页
Journal of Changzhi Medical College
关键词
胃癌
P15
免疫组织化学
淋巴结转移
primary gastric carcinoma
P15
immunohistochemistry
lymph node
