摘要
从淀粉加工厂附近的土壤中分离出一株产耐热、嗜碱性支链淀粉酶的菌株 ,初步鉴定为嗜碱芽孢杆菌 SX- 12 .优化后的最佳发酵培养基为 :可溶性淀粉 3.0 g,蛋白胨 1.0 g,酵母膏 0 .5 g,K2 HPO4 0 .2 g,Mg SO4 · 7H2 O 0 .0 5 g,最适 p H 8.5 ,最适温度 4 0℃ .酶活从最初的 2 .4 u/ m L提高到 4 .6 2 u/ m L .酶的最适 p H 9.0 ,最适温度 5 5℃ .Ca2 + ,Mn2 + ,Mg2 + 等离子是酶的激活剂 ,Zn2 + ,Hg2 + 等离子是抑制剂 .
A bacteria-producing amylopectase was isolated from soil and identified to be Bacillus sp. The fermentation medium and culture conditions of the strain were investigated. The optimal mediums for fermentation are soluble starch 3.0 g, pepton 1.0 g,yeast extract 0.5 g, K 2HPO 4 0.2 g, MgSO 4·7H 2O 0.05 g.The optimum temperature is 40℃. The optimum pH is 8.5. The enzyme activity was increased from 2.4 to 4.62 u/ mL. The optimum temperature for enzyme is 55 ℃. The optimum pH is 9.0. The enzyme activity was promoted by Ca 2+,Mn 2+,Mg 2+, but inhibited by Zn 2+,Hg 2+.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第6期80-85,共6页
Journal of Lanzhou University(Natural Sciences)
基金
中国科学院寒区旱区环境与工程研究所冰芯室创新工程 (2 10 5 0 6 )资助项目
