摘要
通过紫外诱变获得两株稳定的黑曲霉营养缺陷型Ｍｕ２（Ｍｅｔ－）和Ｄ１２（Ｃｙｓ－），并首次证实低温黑暗保存数小时对黑曲霉突变株具有稳定作用。系统地研究了菌龄、渗透压稳定剂、温度、酶的浓度和酶解系统等因素对黑曲霉原生质体形成的影响．采用２％溶壁酶加０．５％纤维素酶，获得了大量的原生质体。在显微镜下观察原生质体形成和再生过程， Ｍｕ２和 Ｄ１２再生率分别为 ５４％和 ３３％。原生质体在正弦交变电场（１ＭＨｚ，４５０ Ｖ／ｃｍ）作用下形成珠串，在高压电脉冲（强度 ９．０ ｋＶ／ｃｍ、时程 ２５ μｓ）触发下，发生融合。
Two stable Aspergillus niger auxotrophs were obtained by using the ultraviolet rays. The fact that the mutants preserving in the dark and low temperature are stable was verified for the first time. The effects of factors such as mycelial ages, osmotic stabiler, temperature, concentrations and kinds of different enzymes on the release of protoplasts were investigated systematically. High yield of protoplasts from two A. Niger auxotrophs Mu2 and D,, was obtained by using a combined enzyme system containing 2% lywallzyme plus 0. 5% cellulase. Morphology of the protoplasts and the regenerated mycelia were also observed under microscope. The fusion of protoplasts from A. niger Mu2 and D12 was induced by the electrical field. Through the dielectrophoresis in non-uniform alternating electric field (1 MHz, 450 V/cm), the protoplasts formed the chains along the direction of the field lines. Electrofusion of protopastsl was triggered by an electric pulse with higher strength (9 .0 kV/cm) and shorter duration (25 μs).
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1991年第3期106-112,共7页
Journal of Tsinghua University(Science and Technology)
关键词
黑曲霉
原生质体
电融合
Aspergillus niger, auxotroph, protoplast electrofusion
