摘要
对经肾活检确诊的30例肾炎病人应用单克隆抗体、间接免疫酶标技术检测肾组织中的前-S_2(Pre-S_2)蛋白,同时对肾脏中的HBsAg、HBeAg及血清HBsag、HBeAg、抗HBs、抗HBe、抗HBc进行了观察。结果显示,7/22例原发性肾炎、6/8例狼疮性肾炎病人的肾小球中有Pre-S_2蛋白沉积,分布于毛细血管袢和(或)系膜区,其分布与肾炎类型、肾组织中免疫球蛋白、补体沉积的多少有关。肾脏中Pre-S_2蛋白的沉积与相同部位HBsAg的沉积有联系,与HBeAg的沉积无关。膜性肾病时,Pre-S_2蛋白的沉积与病毒血症有良好的相关性。狼疮性肾炎时Pre-S_2蛋白的沉积很可能是非特异性的。
The presence of pre-S2 protein in the kidney was investigated in 30 patients with biopsy-proved glomerulonephritis (GN) using indirect immunoperoxidase technique and monoclonal antibody. HBsAg and HBeAg in the kidney were detected using the same techniques and HBsAg, HBeAg, anti-HBs, anti-HBe and anti-HBc in serum were assayed at the same time.It was shown that pre-S2 prtein was present in the kidney in 7/22 patients with primary GN and 6/8 with lupus nephritis. It was deposited along the glomerular capillary walls or within the mes-angium, depending on the type of GN as well as the deposition of immunoglobulins and complement components in the kidney. Deposition of pre-S2 protein in renal tissue was correlated with HBsAg deposition in the glomeruli, but not HBeAg deposition. In membranous GN, there was a good correlation between the presence of pre-S2 protein in the kidney and virus antigetiemia. It is concluded that pre-S2 protein detection in the kidney is of value in evaluating the hepatitis B virus associated GN.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1991年第3期161-164,共4页
Medical Journal of Chinese People's Liberation Army
关键词
乙肝病毒
前-S2蛋白
肾炎
Pre-S2 protein Hepatitis B virus Kidney glomerulonephritis
