摘要
目的 探讨康莱特注射液诱导人肝癌细胞凋亡的作用 ,为康莱特注射液治疗肝癌提供理论依据。方法 采用免疫组织化学及透射电镜技术观察康莱特注射液对肝癌细胞凋亡的影响。结果 肝癌细胞经康莱特注射液培养 2 4h后 ,其凋亡的百分率明显高于对照组和空白组 ,有显著性差异 (P <0 .0 5 ) ,且细胞凋亡在 5 0 %以上者占 44.12 % (15 /3 4 ) ,而对照组及空白组无 1例凋亡超过 5 0 % ;电镜下可见较为典型的细胞凋亡的形态学变化 ,细胞核固缩 ,染色质凝集 ,呈新月型紧贴于核膜周边 ,核碎裂 ,凋亡小体形成等。结论 康莱特注射液能诱导肝癌细胞发生凋亡 ;诱导癌细胞凋亡可能是康莱特注射液治疗作用的重要机制。
Objective To investigate the effect of Kanglaite injection in inducing apoptosis of human hepatocellular carcinoma cells.Methods The hepatocellular carcinoma cells,isolated from 34 surgical specimens of hepatocellular carcinoma,were cultured with kanglaite for 24 hours in vitro.The apoptosis of the cells was observed by immunohistochemical method and electromicroscope.Results The apoptosis rate in kanglaite group was significantly higher compared with control(P<0.05).The characteristics of apoptosis such as the formation of cell-membrane blebs and apoptotic bodies,were observed morphologically by electromicroscope.Conclusion Kanglaite injection could induce apoptosis of hepatocellular carcinoma.The induction apoptosis may be the major mechanism in therapy of hepatocellular carcinoma with Kanglaite injection.
出处
《实用癌症杂志》
2001年第4期343-344,347,共3页
The Practical Journal of Cancer
基金
广西壮族自治区卫生厅资助 (No .Z982 8)
