摘要
用响应面分析法 (RSA)对竹叶黄酮糖苷 (Ebl971)的水解工艺进行了优化。以水解得率为响应值 ,确定在甲醇体系中最适的水解参数为 :底物浓度 1mg/ml,盐酸浓度 1mol/l和水解时间 55min。用此优化条件重复试验 2次 ,平均水解得率为 55.0 % ,水解产物中总黄酮苷元的含量为 56 .6 % ,水解完全 。
The hydrolytic technology of flavone glycoside of bamboo leaf (Ebl 971 )was optimized by RSA method.Taking hydrolysate yield as response value,the optimizing parameters in methanol were figured out as follows:l mg/ml substrate concentration,lmol/l HCl and 55min hydrolysis time.Twice repeat experiments were conducted under this condition and 55.0% mean hydrolysate yield was gained.This hydrolysate contained 56.6% flavone glycone.Results showed that flavone glycoside in Ebl 971 was hydrolyzed completely,and the lipophilicity of this hydrolysate has been improved significantly. ]
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2001年第3期34-37,共4页
Journal of the Chinese Cereals and Oils Association
基金
浙江省高校重大科技攻关项目
省教委科研项目经费资助
关键词
竹叶
黄酮糖苷
提取物
水解苷元
抗氧化性
酸水解
响应面分析
亲油性
食品添加剂
Key words flavone glycoside of bamboo leaf,hydrolytic glycone,acid hydrolysis,response surface analysis(RSA),lipophilicity
