摘要
目的观察来氟米特（Ｌｅｆ）对四氯化碳（ＣＣｌ４）诱导的肝纤维化的预防作用，并对其机制进行探讨。方法采用昆明种小鼠皮下注射ＣＣｌ４制备实验性肝纤维化模型，并同时用Ｌｅｆ进行预防给药，９０ ｄ后进行病理学检查，以及血清中丙氨酸氨基转移酶（ＡＬＴ）、天门冬氨酸氨基转移酶（ＡＳＴ）、一氧化氮（ＮＯ）和透明质酸（ＨＡ）和肝组织中羟脯氨酸（Ｈｙｐ）的生化检查。结果Ｌｅｆ（４，１２，３６ ｍｇ·ｋｇ－１）能降低实验性肝纤维化小鼠血清中升高的ＡＬＴ、ＡＳＴ、ＮＯ、ＨＡ水平和过高的肝组织中Ｈｙｐ的含量。病理组织学检查表明，Ｌｅｆ（４，１２，３６ ｍｇ·ｋｇ－１）对实验性肝纤维化有明显的预防作用。结论Ｌｅｆ对实验性肝纤维化预防作用可能是通过降低ＮＯ的水平，进而降低细胞毒作用和纤维化进程而产生的。
AIM To observe the preventive action of leflunomide (Lef) on CCl4-induced liver fibrosis and explore its mechanism. METHODS Model of liver fibrosis in mice was induced by subcutaneous injection of CCl4(20% ). The levels of ALT\AST.NO in plasma and Hyp in liver tissue were determined by spectroscopy. The content of HA in plasma was determined by radioimmunoassay. RESULTS Pretreatment of Lef (4, 12, 36 mg. kg- 1 ) significantly reduced the elevated levels of ALT.AST.NO in plas- ma and Hyp in liver tissue, Pathological examination suggested Lef has preventive action on experimental liver fibrosis with significance. CONCLUSION Lef has significantly preventive action on CCl4-induced liver fibrosis possibly mediated by reduction of NO.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第2期203-205,共3页
Chinese Pharmacological Bulletin
