摘要
为进一步探讨中药“热毒清”拮抗矽尘毒性效应,对经提纯的不同培养阶段五组小鼠腹腔巨噬细胞用细胞化学和扫描电镜法进行了动态观察。结果:随培养时间和机能状态不同,五组细胞呈不同的外形和一系列表面超微结构变化特征。SiO_2、中药、P_(204)及中药加P_(204)组细胞于5分钟内吞噬SiO_2粉尘,观察了细胞对SiO_2的反应方式及各不同吞噬阶段细胞的表面变化特征。中药组的细胞表面超微结构变化与正常组相似。SiO_2组细胞吞噬SiO_2后远较中药组迅速死亡、崩解,且细胞内ACP和SDH活性变化远低于中药组。认为,中药“热毒清”有明显的抗矽尘毒性效应。
In order to elucidate further the antitoxic effect of chinese medicinal preparation-'Re Du Qing' on silica particles, The purified peritoneal maerophages at various cultural stages from five groups of mice were observed dynamically both by routine cytochemical morphological study and by scanning electron microscopy looking for cell surface ultrastructural changes. The results showed that each group had its characteristics of cell outline and series of cell surface ultra struetural changes appeared In different stages of cultured and various functional stages. The macrophages from the treatment of silica. chinese medicinal preparation, P_(204) and chinese medicinal preparation plus P_(204) groups appeared to be able to phagocytose the silica particles within 5 minuts, and their reactionary forms to silica particles and the characteristic cell surface changes in various stages of phagoeytosis were observed, The cell surface ultrastruetural changes of the chinese medicine group were similar to that of the normal one. The peritoneal macrophages in the silica group having phagocytosed silica particles showed a rapid death and breaking down much earlier than that chinese medicine group. The activities of the intracellular ACP and SDH in the silica group were also much lower than that of the latter one. It suggested that the chinese medicinal preparation-'Re Du Qing' was evidently related to the antitoxic effect on silica particles.
出处
《职业医学》
CAS
1989年第2期6-7,I001,共3页
China Occupational Medicine
关键词
二氧化硅
中药
“热毒清”
巨噬细胞
细胞培养
扫描电镜
矽尘
拮抗
silica, chinese medicinal preparation-'Re Du Qing,' macrophage, cell culture, scanning electron microscope.
