摘要
以烤烟品种Hicks和SpeightG 2 8为材料 ,针对烟草RAPD分析中PCR过程的Taq酶浓度、Mg2 +浓度、退火温度、循环数、Primer浓度、dNTP浓度等因素进行了研究。结果表明 ,体系中含 0 .8UTaq酶 ,4 0mmol/LMgCl2 ,10 μmol/L引物 ,3.0mmol/LdNTP ,循环数为 4 0 ,退火温度 36℃效果较好。从而确立适合烤烟RAPD分析的PCR程序主要条件 。
With tobacco variety Hicks and Speight G 28 as experimental materials, taqase concentration,Mg 2+ concentration,annealing temperature,number of amplified cycles,primer concentration and dNTP concentration of RAPD analysis were studied. The results indicated that under the condition of 0.8 U taqase, 40 mmol/L MgCl 2, 10 μmol/L primer, 3.0 mmol/L dNTP, annealing temperature at 36 ℃and 40 cycles, the effect was very good. RAPD pattern founded in this experiment layed good foundations for application of RAPD analysis in tobacco variety identification and germplasm research.
出处
《中国烟草科学》
CSCD
2001年第1期37-40,共4页
Chinese Tobacco Science
基金
云南省烟草公司资助项目! (NO .983A0 7)
