摘要
为了充分利用雨生红球藻这一微藻资源,研究虾青素低温抗氧化的萃取技术。本文利用扫描电镜(scanning electron microscopy,SEM)研究了雨生红球藻粉超微粉碎后细胞壁的结构变化,确认破壁的最佳条件。采用超临界CO2对其进行萃取,运用响应面法(response surface methodology,RSM)优化萃取工艺条件,并对萃取后的精油逐个进行GC-MS分析其脂肪酸成分。结果表明最佳工艺条件为:原料粒度60目,萃取压力42 MPa,萃取温度47℃,萃取时间120 min,液固比1.6,在此条件下,虾青素在藻粉中的提取率可达1.23%,在萃取物中提取率为4.84%。然后将响应面不同条件得到的萃取物进行GC-MS分析,可知当压力40MPa,温度55℃,液固比为1,不饱和脂肪酸(UFA)含量最高为155.1 mg·g-1;当压力45MPa,温度45℃,液固比为2,多不饱和脂肪(PUFA)含量最高为132.05 mg·g-1。
In order to make full use of Haematococcus pluvialis, antioxidant extraction temperature was studied. By using the scanning electron microscopy （SEM）, this changes in the cell wall after the superfine grinding of Haematococcus pluvialis and technology of axtaxanthin in low study investigated the structural determined optimal conditions of destroying the cell wall. Super-critical CO2 extraction of astaxanthin was optimized by response surface methodology （RSM）. The essential oil were identified by GC-MS. The optimal extraction conditions were as follows ： the particle size for 60 screen mesh, liquid-solid ratio 1.6, pressure 42MPa, temperature 47℃, extracting time 120min. under those conditions, the extraction yield of astaxanthin from algae was 1.23% , from the extract was 4. 84%. GC-MS analysis of essential oil indicated that the content of unsaturated fatty acids （UFA） reached the maximum value of 155. 1 mg·g^-1 under the extraction conditions： liquid-solid ratio was 1, pressure 40MPa, temperature 55℃. Meanwhile, under conditions of liquid-solid ratio as 2, pressure 45MPa, temperature 45℃, the content of Polyunsaturated fatty acids （PUFA） reached maximum value of 132.05 mg·g^-1.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第6期1052-1061,共10页
Journal of Nuclear Agricultural Sciences
基金
国际合作项目(S2011ZR0360)
