摘要
分别用浓度0.02、0.04、0.06、0.08和0.10g/L的蓖麻毒蛋白对泥鳅进行急性毒性试验,在染毒处理第1、3、7、11d时将泥鳅断尾制血涂片,观察红细胞的凋亡情况.结果表明:蓖麻毒蛋白对泥鳅红细胞的凋亡具有明显的浓度和时间依赖性;染毒初期,泥鳅红细胞的凋亡率随蓖麻毒蛋白浓度的升高而不断增加;随染毒处理时间的延长,凋亡率曲线达峰值,随后凋亡率曲线不断降低,整个凋亡率曲线呈抛物线形.结论:蓖麻毒蛋白能诱导泥鳅红细胞凋亡.
An acute toxicity test of ricin on loach red cells was conducted by applying solutions of different ricin concen- trations at 0.02, 0.04, 0.06, 0.08 and 0.10 g/L. On the 1st, 3rd, 7th and, 11th days, the loach tails were cut off and blood smears were made to find about the red cell apoptosis. The findings showed that： the apoptosis of ricin on erythrocytes of loach was marked by an obvious time and concentration dependency. At the early stage of the toxicity test, the red cell apoptosis rate increased as the riein concentration aseended; yet with the extension of exposure time, the apoptosis rate curve reached its peak value, then the apoptosis rate decreased persistently with the overall apoptosis curve taking the shape of a parabola.. Conclusion： ricin can induce apoptosis in erythrocytes of loach.
出处
《内江师范学院学报》
2014年第6期44-46,共3页
Journal of Neijiang Normal University
基金
四川省教育厅自然科学类重点资助项目(07ZC032)
内江师范学院大学生科研项目(12NSD-43)
