摘要
为探讨HIV -1gp41N端融合肽诱导膜融合的机理 ,利用傅里叶变换红外光谱技术研究了化学方法合成的代表HIV -1gp41N末端的23肽 (HIVWT)分别与酸性POPG和中性POPC脂膜作用后 ,多肽H/D交换程度的变化及其在膜中的取向。结果表明 ,HIVWT 分子中的大部份氨基酸残基被POPG脂膜保护 ,分子骨架上的酰胺质子只有11.9 %可被D交换 ,而HIVWT 被POPC脂膜保护的程度则小得多 ;在POPG脂质体中 ,HIVWT 分子中的α -螺旋与脂双层平面的角度为29±2°,β-折叠与脂双层平面的夹角为25±1°。在POPC脂质体中 ,HIVWT 分子的α -螺旋与脂双层平面的角度为22±1°;β-折叠与脂双层平面的夹角为26±3°。根据实验结果 ,对HIVWT 的插膜状态进行了讨论。
To investigate the mechanism of membrane fusion induced by HIV-1 gp41 N terminal fusion peptide, we studied its membrane insertion state and orientation by H/D exchange and polarized FTIR in acidic POPG and neutral POPC liposomes, respectively. The results indicate that only 11.9% of backbone amide protons of HIVWT in POPG vesicles undergo H/D exchange, whereas 44.6% amide protons are exchangeable for HIVWT in POPC SUV. The average angles of α-helix and β-strand of HIVWT with respect to the POPG bilayer surface are 29±2° and 25±1°, respectively. In the presence of POPC SUV, the average angle between the α-helix of HIVWT and the bilayer surface is 22±1°, and the average angle of β-strand of HIVWT is 26±3°. The membrane insertion state of HIVWT was discussed.
出处
《生物物理学报》
CAS
CSCD
北大核心
2001年第1期46-52,共7页
Acta Biophysica Sinica
基金
国家自然科学基金!39770181资助
