摘要
目的:观察黄芪和地黄共同促人脐静脉内皮细胞血管新生的作用,并初步探讨其作用机制。方法:分别考察黄芪和地黄的混合提取物浓度为18.75,37.5,75mg/L,采用MTT法检测内皮细胞的增殖状况,通过划痕实验和Transwell实验考察内皮细胞的迁移能力,小管形成实验观察新生血管的形成,免疫印迹测定VEGFR2、Akt、p38、FAK的总蛋白及其磷酸化的表达。结果:药物浓度18.75mg/L不能提高体外成血管能力;37.5mg/L能促进内皮细胞增殖及成血管,并能显著提高p-FAK和p-VEGFR-2的表达,但不能促进内皮细胞迁移;75mg/L能促进内皮细胞增殖、迁移及成血管,并能显著提高p-Akt、p-FAK和p-VEGFR-2的表达。结论:黄芪和地黄诱导内皮细胞增殖、迁移和血管形成,促进血管新生,其机制可能与VEGF-VEGFR-2通路有关。
Objective： To investigate the effects and mechanism of extract of radix astragali and radix reh- manniae on human umbilical vein endothelial cells （HUVECs） in promoting angiogenesis. Methods. Methyl thiazolyl tetrazolium （MTT） assay, wound healing assay and Transwell chamber migration as- say,and in vitro tube formation assay were used to test proliferation, migration and angiogenesis capaci- ties of HUVECs respectively. Total protein and phosphorylation of VEGFR-2, Akt, p38 and FAK were detected by Western blot. Results： The compounds of radix astragali and radix rehmanniae at 37.5 mg/L promoted cell proliferation and angiogenesis and significantly increased the expression of p-FAK and p-VEGFR-2. Cell proliferation, migration and angiogenesis, as well as the expression of p-Akt, p- FAK and p-VEGFR-2, were enhanced by the compounds at 75 g/L. Conclusion： Radix astragali and radix rehmanniae extract could induce HUVECs proliferation, migration and angiogenesis, and the mechanism may be partly due to the up-regulation of VEGF-VEGFR-2 pathway.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2014年第3期370-374,共5页
Medical Journal of Wuhan University
关键词
黄芪
地黄
血管新生
伤口愈合
Radix Astragali
Radix Rehmanniae
Angiogenesis
Wound Healing
