摘要
本研究建立了反转录聚合酶链反应 ( RT PCR)技术检测猪瘟病毒 ( Hog Cholera Virus,HCV)方法。合成的二对引物 HCV 1 / HCV 2和 HCV A1 / HCV A2成功地扩增兔化弱毒株、法国株和石门株的核酸 ,并对采自博白县 3个、贵港市 1个和武宣县 1个猪场共 1 3份病料进行检测。结果从博白县和贵港市的 1 1份材料中检测到 HCV的核酸。
In this study reverse transcription coupled with the polymerasechain reaction(RT-PCR) has been established to detect hog cholera virus (HCV). Two pairs of primers,HCV-1/HCV-2 and HCV-A1/HCV-A2,has been synthesized and successfully to amplify nucleic acids of HCLV strain, France strain and Shimen strain of HCV. In experiment proper,13 clinical samples were examined from three differen t locolities in Guangxi namely, Bobai, Guigang and Wuxuan constituting three, one and one pig farms respectively. As a result, 11 of 13 samples from Bobai and Guigang by both primers indicating that the sick pigs in which the sample was derived were infected by HCV.
出处
《广西农业生物科学》
CAS
CSCD
2001年第1期17-20,共4页
Journal of Guangxi Agricultural and Biological Science
基金
广西大学博士科研启动基金资助项目! ( X9830 3)
广西区教育厅资助项目! ( T981 0 6)
关键词
反转录-聚合酶链反应
猪瘟病毒
检测
reverse transcription
polymerase chain reaction (PCR)
hog cholera virus (HCV)
