摘要
通过RT-PCR和RACE技术从新疆海岛棉品种新海14号中克隆得到了一个MFT(MOTHER OFFT AND TFL1)类似基因,命名为GbMFT2基因(GenBank登录号为KF739071)。GbMFT2基因的开放阅读框(ORF)为519 bp,编码172个氨基酸,含有一个磷脂酰乙醇胺结合蛋白(PEBP)结构域。GbMFT2基因和GbMFT1基因的核苷酸相似性仅为56.7%,二者蛋白的相似性为62.7%。系统进化树分析表明GbMFT2编码产物属于MFT-like亚家族。qRT-PCR分析表明,GbMFT2基因在不同的组织中均有表达,在胚珠和茎中表达量较高。半定量RT-PCR结果表明,和GbMFT1基因的表达模式不同,GbMFT2基因在刚萌发的种子中的表达量很低;但随着ABA浓度的升高表达量显著提高,并且ABA处理下萌发24 h的种子中的表达量要高于72 h中的表达量,表明该基因的表达受ABA的调节,可能在种子萌发过程中起重要作用。
The phosphatidylethanolamine-binding protein（PEBP） gene family is present in angiosperms with three subfamilies,FT-like,TFL1-like and MFT-like,which having been shown to act as key regulators to control growth and differentiation.Compared with the other two kinds,the exact molecular function of MFT-like subfamily genes is still not clear.One MFT（MOTHER OF FT AND TFL1）-like homolog was isolated from Gossypium barbadense L.cv.Xinhai 14 through RT-PCR and RACE method,which was designated GbMFT2 gene（GenBank accession number：KF739071）.The open reading frame（ORF） of GbMFT2 is 519 bp in length,encoding a putative protein of 172 amino acids,containing a PEBP conserved motif.Sequence alignment showed that the nucleotide of GbMFT2 gene has only 56.7％ similarity with GbMFT1 gene and the amino acids of GbMFT2 protein has 62.7％ similarity with GbMFT1 protein.Phylogenetic analysis revealed that GbMFT2 belong to the MFT-like clade.Expression analysis by qRT-PCR indicated that GbMFT2 displayed a much broader expression range,with a maximum expression in stem and ovule.Expression analysis by semi-quantitative RT-PCR method indicated that GbMFT2 expression levels in germinating seeds were low,but gradually elevated when treated along with increased concentrations of ABA.Our research showed that the expression of GbMFT2 was up-regulated by ABA in germinating seeds,showing significant differences compared with GbMFT1 in the case of ABA treatment.
出处
《棉花学报》
CSCD
北大核心
2014年第3期197-203,共7页
Cotton Science
基金
新世纪优秀人才支持计划项目(NCET-12-1072)
新疆生产建设兵团博士基金项目(2012BB007)
国家自然科学基金(31360366)
