摘要
应用整体化色谱柱，反相条件下对18H-甘草次酸差向异构体的拆分进行了探讨。采用Chromolith RP-18e整体化色谱柱，乙酸铵-乙腈-甲醇三元流动相体系，考察了乙酸铵浓度、有机添加剂含量、柱温等因素对甘草次酸差向异构体拆分的影响，在优化的色谱条件下，甘草次酸差向异构体达到了基线分离，分离度可达5．30。18α-甘草次酸与18β-甘草次酸分别在2．16—23．76μg／mL与4．24—46．64μg／mL范围内呈良好的线性关系。建立的方法简便快速，可应用于甘草次酸类药物的质量监测与控制，以及甘草次酸异构体的转化评价。
The separation of the epimers of 18H-glycyrrhetinic acid by the reversed HPLC with a monolithic column was investigated. A Chromolith RP-18e column was used, and ammonium acetate-acetonitrile-methanol ternary system was used as the mobile phase. The effects of the concentration of ammonium acetate, the content of organic modifiers and the column temperature on the separation of the epimers of glycyrrhetinic acid were investigated. Baseline separation was achieved, and the resolution could reach 5.30 under the optimized condition. The calibration curves were linear for 18α- and 18β-glycyrrhetinic acid in the range of 2. 16 × 23.76 μg/mL and 4. 24 × 46. 64 μg/mL, respectively. The method for the separation of the epimers of glycyrrhetinic acid was convenient and rapid, and can be used to monitor the quality of glycyrrhetinic acid.
出处
《化学试剂》
CAS
CSCD
北大核心
2014年第6期560-562,共3页
Chemical Reagents
基金
福建省自然科学基金杰出青年基金项目(2012-J06005)
福建省高等学校新世纪优秀人才支持计划(JK-2011030)
