摘要
Fibrillin 11（FBN 11）是植物质体中FBN蛋白家族的重要成员之一,比其他成员多300～500个氨基酸残基,说明其可能存在某些特异性结构和功能。本研究在水稻幼苗中扩增获得到了一个受非生物逆境胁迫诱导的OsFBN11基因的全长cDNA,该基因含有12个内含子和13个外显子。其编码蛋白的分子量为72.36 kD,pI值为9.26。生物信息学分析结果表明,该蛋白含有无规则卷曲、α螺旋和β-折叠3种氨基酸二级结构,不含有跨膜结构域,蛋白亲水性强,PSORT软件预测该蛋白可能定位于叶绿体中。进一步对14种植物FBN11蛋白的同源性和5个物种该蛋白的保守结构域分析,发现该蛋白兼有典型的PAP-Fibrillin结构域和蛋白激酶PKc结构域。水稻全生育期芯片分析显示该基因主要在愈伤组织、叶片和根系中高水平表达。RT-PCR检测结果显示,该基因在水稻幼苗中受ABA、NaCl和干旱胁迫处理上调表达。上述结果表明,OsFBN11可能在水稻质体发育和抗非生物胁迫反应中发挥重要作用。
Fibrillin（ FBN11） is one of the important members of the Fibrillin（ FBN） proteins family in plastids of plants. It has 300-500 amino acid residues longer than other members in this family,suggesting its specific functions in higher plants. In this study,the full-length cDNA of OsFBN11 gene regulated by abiotic stresses in rice seedlings was cloned and analyzed,and it was found that this gene contained 12 introns and 13 exons,encoding a protein with a calculated molecular mass of 72. 36 kD and a pI value of 9. 26. Bioinformatics analysis showed that OsFBN11 was a hydrophilic protein without transmembrane domain. The secondary structure was composed of random-coil,alpha helix,and extended strand. PSORT software predicted this protein might be localized in chloroplast. Analyses of FBN11 protein homologies in 14 plant species and conserved domains in 5 plant species indicated that this protein consisted of a typical PAP-Fibrillin domain and PKc kinase domain. The chip analysis of whole growth period in rice revealed that OsFBN11 gene was highly expressed in callus,leaves,and root tissues. RT-PCR analysis demonstrated that OsFBN11 was up-regulated by ABA,NaCl,and drought stresses. The results above suggested that OsFBN11 might play an important role in plastid development and responses to abiotic stresses in rice.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第3期655-661,共7页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31371550)
中央高校基本科研业务费专项资金(2012MBDX008)
华中农业大学自主科技创新基金(2012SC14)
