摘要
目的探讨氧化型低密度脂蛋白(ox-LDL)对急性冠状动脉综合征(ACS)患者外周血CD4+CD25+调节性T细(Tregs)表达的影响。方法收集健康对照组30例,男16例,女14例,平均年龄58.45±5.98岁;稳定型心绞痛(SA)患者23例,男12例,女11,平均年龄59.27±6.93岁;急性冠状动脉综合征患者45例,其中不稳定型心绞痛(UA)患者27例,男14例,女13例,平均年龄62.33±7.32岁,急性心肌梗死(AMI)患者18例,男11例,女7例,平均年龄65.71±8.12岁,外周血标本,通过ELISA检测血浆ox-LDL、IL-10表达水平,通过流式细胞术检测各组患者Tregs表达水平。密度梯度离心法分离外周血淋巴细胞,用ox-LDL孵育淋巴细胞48 h后流式细胞术检测Treg表达水平。结果与健康对照组和SA组相比,ACS患者外周血ox-LDL水平显著升高(P<0.05),而IL-10和Tregs表达水平显著下降(P<0.05),ox-LDL与Tregs水平呈明显的负相关(r=-0.72,P<0.01),与IL-10也呈明显的负相关(r=-0.516,P<0.01),同时ox-LDL作用于外周血淋巴细胞48 h后,Tregs水平明显下降。结论 oxLDL可以下调ACS患者Tregs的表达,减弱Tregs的保护作用,进一步促进动脉粥样硬化进展。
Aim To investigate the effects of oxidized LDL （ox-LDL） on peripheral blood level of regulatory T cells （Tregs） in patients with acute coronary syndrome （ACS）. Methods 18 patients with acute myocardial infarction （AMI）, 27 patients with unstable angina （UA）, 23 patients with stable angina （SA） and 30 healthy subjects （NC） were recruited. The plasma levels of ox-LDL and IL-10 were measured by ELISA, and the percentage of Tregs in peripheral blood were detected by flow cytometry. Peripheral blood mononuclear cells （PBMC） were prepared by Ficoll density gradient, then were incubated with ox-LDL for 48 h for analysis of flow cytometry. Results The frequencies of Tregs and level of IL-10 were significant lower in AMI and UA patients than those in the SA and NC groups, while the level of ox-LDL was elevated markedly in patients with AMI and UA compared with SA and NC group. In addition, the levels of plasma ox-LDL were negatively correlated with the levels of Tregs and IL-10. Moreover, the peripheral blood level of Tregs was obviously decreased after incubation with ox-LDL for 48 h. Conclusion ox-LDL could down-regulate the peripheral blood level of Tregs in patients with ACS, thereby promoting the progression of ACS.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第3期293-297,共5页
Chinese Journal of Arteriosclerosis
