摘要
用二甲基亚砜(DMSO)替代甘油做猪精液冷冻保护剂并对其效果进行研究。以3%甘油作为对照组,比较不同浓度的DMSO(40、50、60、70、80mL/L)对猪精液冷冻保护效果的影响,从而得到DMSO的最佳添加量。同时,将甘油和DMSO配伍(4∶1、3∶1、3∶2、1∶1和2∶3)成最终浓度为3%的混合冷冻保护剂,并分别记作A、B、C、D和E组,对比其对猪精液冷冻保存的效果,筛选出最佳配比。结果显示,添加6%的DMSO,解冻后精子畸形率、质膜完整率和顶体完整率均明显优于对照组和其它DMSO添加组(P<0.05),分别为16%、49%和51%,解冻后精子活率为26%显著高于其它DMSO添加组(P<0.05),但低于对照组(P<0.05)。B组解冻后精子畸形率为13%,显著低于C组、D组、E组、4%、5%、7%、8%DMSO添加组和对照组(P<0.05),但与A组、6%DMSO添加组差异不显著(P>0.05),顶体完整率和质膜完整率分别为53%和52%,均显著高于40、50、70、80mL/L DMSO添加组和其他配伍组(P<0.05),但与6%DMSO添加组差异不显著(P>0.05),解冻后精子活率37%显著低于对照组(P<0.05),但显著高于其他DMSO添加组和混合配伍组(P<0.05)。所以,DMSO单独添加时,最佳添加量为60mL/L,而最终浓度为30mL/L的混合冷冻保护剂最佳配比是甘油与DMSO为3∶1。
This experiment was the study on the effects of dimethyl sulfoxide （DMSO）on boar semen cryo-preservation instead of glycerin.With the control group of 30 mL/L glycerin,by comparing the effects of different groups of DMSO （40,50,60,70,80 mL/L）on boar semen cryopreservation,the DMSO optimum concentration was selected.At the same time,the compatibility of glycerin and DMSO （4∶1,3∶1,3∶2, 1∶1 and 2∶3）were conducted into mixed cryoprotectants with the final concentration of 30 mL/L,respec-tively marked for group A,B ,C,D and E,and then the boar semen cryopreservation were compared and the optimum proportion were selected.The results showed that by adding 60 mL/L DMSO,after being frozen-thawed,the sperm abnormal rates,the plasma membrane integrity and sperm acrosomes integrity were remarkably better than that in the control group and other groups （P 〈 0.05）added with DMSO, which were 16%,49% and 51%,while the sperm viability was 26%,which was higher than that in other groups （P 〈 0.05）added with DMSO but lower than that in the control group （P 〈 0.05）.The sperm abnormal rate of Group B was 13% after being frozen-thawed,remarkably lower than that in groups C,D, E added with 40,50,70,80 mL/L DMSO and the control group （P 〈 0.05）,but compared with group A and the group added with 60 mL/L DMSO（P 〉 0.05）,it had no significant difference.The sperm acro-somes integrity and the plasma membrane integrity were 53% and 52%,which were remarkably higher than groups added with 40,50,70,80 mL/L DMSO and other compatible groups （P 〈 0.05）,but had no significant difference with the group added with 60 mL/L DMSO （P 〉 0.05）.The sperm viability that was 37% was remarkably lower than that in the control group （P 〈 0.05）,but remarkably higher than that in other DMSO added groups and the compatible groups （P 〈 0.05）.So when adding DMSO sepa-rately,the optimum concentration was 60 mL/L,and the best proportion for the mixed cryoprotectants a-gent,of which the final concentration was 30 mL/L,was 3∶1 （glycerin to DMSO）.
出处
《动物医学进展》
CSCD
北大核心
2014年第5期56-61,共6页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31372280)
关键词
二甲基亚砜
猪精液
冷冻保存
最佳配伍
DMSO （dimethyl sulfoxide）
boar semen
cryopreservation
optimum compatibility
