摘要
目的观察微小RNA（miRNA）-375在食管鳞状细胞癌（ESCC）中的表达，探讨其启动子区的甲基化水平与ESCC发生的关系。方法对62例ESCC组织标本和正常食管组织（距癌肿边缘〉3cm）进行实时荧光定量聚合酶链反应（FQ—PCR）检测，使用甲基化特异性聚合酶链反应（MSP）检测样本中miRNA-375上游启动子区的甲基化。统计学分析miRNA-375的表达与临床因素的相关性。结果62例ESCC组织标本中有53例miRNA．375E呈明显低表达（85％）。MSP结果显示，ESCC组织的甲基化水平明显高于正常食管组织，差异有统计学意义（P〈0．05）。与临床因素的相关分析显示，miRNA-375在ESCC组织中低表达，与ESCC的TNM分期呈负相关（P〈0．05）。结论ESCC组织中存在miRNA-375基因异常低表达及其启动子区的高甲基化，可能在ESCC发生发展中发挥重要作用。
Objective To investigate the expression of microRNA （miRNA）-375 and its genomic promoter DNA methylation in esophageal squamous cell carcinoma （ESCC）. Methods The expression of miRNA-375 in ESCC and the matched normal esophageal tissues （from the tumor edge 〉 3 cm） was detected by real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）. DNA methylation status was examined by methylation specific polymerease chain reaction （MSP）. The association between miRNA-375 expression and clinical factors were analyzed statistically. Results As compared with normal controls, miRNA-375 was significantly decreased in ESCC. The methylation levels in ESCC tissues were significantly higher than in the matched normal esophageal tissues （P 〈 0. 05 ）. The low expression of miRNA-375 was significandy associated with ESCC TNM stage （P 〈 0. 05）. Conclusion The lower expression of miRNA-375 and aberrant hypermethylation of the CpG island promoters in ESCC may play a crucial role in the carcino- genesis of ESCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第5期1125-1127,共3页
Chinese Journal of Experimental Surgery
