摘要
目的建立HPLC法测定双根口服液中（R，S）-告依春的含量。方法色谱柱为SHIMADZUVP—ODS色谱柱（150mm×4．6mm，5μm），流动相为甲醇-0．025％磷酸溶液（7：93），流速为1．0ml·min^-1，检测波长为245rim，柱温为30℃；自动进样器进样，进样量10μl。结果（R，S）-告依春在1．8794～60．1398μg·ml^-1浓度范围内线性关系良好（r=0．9999，n=6），平均回收率为98．92％，RSD为1．02％（n=9），并依据试验数据，讨论了（R，S）-告依春的含量限度。结论本法操作方便、结果准确，精密度好，专属性强，可用于双根口服液的质量控制。
Objective To establish an HPLC method for determination of （R, S）-epigoitrin in Shuanggen oral liquid. Methods The separation of samples was performed on SHIMADZU VP-ODS （ 150 mm × 4.6 mm, 5 μm） and the mobile phase consisted of methol-0. 025% phosphoric acid solution（7： 93） at a flow rate of 1.0 ml · min^ - 1. The detection wavelength was set at 245 nm, the column temperature was 30 ℃ and the injection volume （ in an automatic sampler） was 10 μl Results （R, S）-epigoitrin showed a good linear relationship within the range of 1. 8794-60. 1398 μg ·ml^-1（ r =0.9998, n =6） with an average recovery of 98.92% （RSD = 1.02%, n =9）. Conclusion This method is easy, simple, accurate and stable quality control of Shuanggen oral liquid. in results with good specificity, which is applicable to
出处
《解放军药学学报》
CAS
2014年第2期167-169,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
