摘要
应用ISSR（inter-simple sequence repeat）分子标记技术对13个不同来源的金福菇（Trichooma lobayense Heim.）菌株进行DNA指纹图谱分析,利用筛选出的7条ISSR引物对金福菇基因组DNA进行ISSR-PCR扩增,共扩增出DNA谱带73条,其中多态性谱带72条,多态位点百分率为98.6％,金福菇各菌株间的遗传相似系数变化范围为0.3425～0.8082.聚类分析结果表明,在遗传相似系数为0.5时,可将13个金福菇菌株划为2大类群.
ISSR（inter-simple sequence repeat） molecular markers were used to analyze DNA fingerprintings of 13 Tricholoma lobayense wildtype suains originating from different locations worldwide.A total of 73 bands were amplified using 7 ISSR primers,of which 72 bands were polymorphic.Perccentage of polymorphic bands was 98.6％.ISSR similarity coefficients ranged from 0.3425 to 0.8082.Cluster analysis further demonstrated that the 13 strains were clustered into two groups at the coefficient of 0.5.
出处
《西南农业学报》
CSCD
北大核心
2014年第2期768-771,共4页
Southwest China Journal of Agricultural Sciences
基金
广西农业科学院基本科研业务专项(桂农科2013YM13)
广西农业科学院基本科研业务专项重点扶持项目(桂农科2013YF03)
关键词
金福菇
ISSR
遗传多样性
Tricholoma lobayense
ISSR
Genetic diversity
