摘要
目的探讨难治性肺炎患儿细胞因子水平变化及意义。方法选取确诊为难治性肺炎患儿97例,根据其肺炎支原体(MP)感染情况分为MP感染组(53例)及非MP感染组(44例);选取同期就诊的支气管异物患儿30例作为对照组。采用双抗体夹心ABC-ELISA法检测患儿肺泡灌洗液(BALF)中IL-6、IL-10水平。结果难治性肺炎患儿BALF中IL-6、IL-10水平高于对照组,差异有统计学意义(P<0.05);MP感染组BALF中的IL-6、IL-10水平高于非MP感染组,差异有统计学意义(P<0.05)。结论 IL-6、IL-10在难治性肺炎患儿,尤其是MP感染的难治性肺炎的免疫功能紊乱中起到一定的作用。
Objective To explore the cytokines levels in bronchoalveolar lavage fluid （BALF） of children with refractory pneumonia and its significance. Methods A total of 97 children with refractory pneumonia were selected and then were divided into Mycoplasma pneumoniae （MP） infection group （53 cases） and non-MP infection group （44 cases） according to MP infec- tion status. Meanwhile, 30 children with bronchial foreign body at our clinic were chosen as controls. ABC-double antibody sandwich ELISA method was used to detect interleukin （IL）-6 and IL-10 levels in BALF. Results The IL-6 and IL-10 levels in BALF of children with refractory pneumonia were significantly higher than those in the control group （P〈0.05）. Furthermore, the IL-6 and IL-10 levels in BALF in MP infection group were significantly higher than those in non-MP infection group （P〈 0.05）. Conclusions The cytokines IL-6 and IL-10 play some role in immune dysfunction induced by refractory pneumonia, es- pecially caused by MP infection.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2014年第4期336-338,共3页
Journal of Clinical Pediatrics
关键词
难治性肺炎
肺泡灌洗液
白介素-6
白介素-10
儿童
refractory pneumonia
bronchoalveolar lavage fluid
interleukin-6
interleukin-lO
child
