摘要
本试验旨在研究复合菌固态发酵培养基及发酵工艺的优化。试验采用DPS软件中的混料试验设计和均匀试验设计方案,共配伍成14种复合菌固态发酵培养基和14种发酵工艺,通过测定发酵固态料中的活菌数及β-葡聚糖、甘露聚糖、有机酸含量筛选优良组方并对发酵工艺进行优化。结果表明:1)优化后复合菌固态发酵培养基为麸皮52.69%、玉米皮20.45%、玉米粉16.07%、米糠4.09%、棉籽粕1.86%、豆粕3.99%、无机盐0.85%;2)发酵工艺优化为含水量60%、pH 8.0、温度30℃、时间46 h,此时活菌数为6.71×108CFU/g、葡聚糖含量为279.83 mg/g、甘露聚糖含量为156.82 mg/g、酒石酸含量为2.78 mg/g、苹果酸含量为8.28 mg/g、柠檬酸含量为1.34 mg/g、丁二酸含量为2.54 mg/g。由此可见,本试验优化了复合菌固态发酵培养基及发酵工艺。
This experiment was conducted to study the optimization of compound bacteria solid-state fermenta- tion medium and fermentation process. The mixing experimental design and uniform experimental design by DPS software were used, combined into 14 kinds of solid-state fermentation medium and 14 kinds of fermenta- tion process. Determining the contents of viable count, β-glucan, mannan and organic acid to filter the fine components and optimized the fermentation process. The results showed as follows： 1 ） the optimized com- pound bacteria solid-state fermentation medium was wheat bran 52.69%, corn bran 20. 45 % , corn flour 16.07% , rice bran 4.09% , cottonseed meal 1.86% , soybean meal 3.99% and inorganic salt 0.85% ; 2 ） the optimized fermentation process was moisture 60%, pH 8.0, temperature 30℃ and time 46 h. The indexes of optimal combination were viable count 6.71 ×10^8 CFU/g, glucan content 279.83 mg/g, mannan content 156.82 mg/g, tartaric acid content 2.78 mg/g, malic acid content 8.28 mg/g, citric acid content 1.34 mg/g and butanedioic acid content 2.54 mg/g. In conclusion, this experiment optimizes the compound bacteria sol- id-state fermentation medium and fermentation process.
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第4期1019-1027,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(31260560)
现代农业(奶牛)产业技术体系建设专项资金资助(CARS-37)
关键词
复合菌
活性物质
发酵培养基
发酵工艺
compound bacteria
active material
fermentation medium
fermentation process
